{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gomez-Gonzalez PJ"],"funding":["Newton Institutional Links","Medical Research Council","Biotechnology and Biological Sciences Research Council"],"pagination":["bbac256"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9487601"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(5)"],"pubmed_abstract":["With >1 million associated deaths in 2020, human tuberculosis (TB) caused by the bacteria Mycobacterium tuberculosis remains one of the deadliest infectious diseases. A plethora of genomic tools and bioinformatics pipelines have become available in recent years to assist the whole genome sequencing of M. tuberculosis. The Oxford Nanopore Technologies (ONT) portable sequencer is a promising platform for cost-effective application in clinics, including personalizing treatment through detection of drug resistance-associated mutations, or in the field, to assist epidemiological and transmission investigations. In this study, we performed a comparison of 10 clinical isolates with DNA sequenced on both long-read ONT and (gold standard) short-read Illumina HiSeq platforms. Our analysis demonstrat"],"journal":["Briefings in bioinformatics"],"pubmed_title":["Portable sequencing of Mycobacterium tuberculosis for clinical and epidemiological applications."],"pmcid":["PMC9487601"],"funding_grant_id":["MR/N010469/1","261868591","MR/M01360X/1","MR/R025576/1","BB/R013063/1","MR/R020973/1"],"pubmed_authors":["Campino S","Phelan JE","Gomez-Gonzalez PJ","Clark TG"],"additional_accession":[]},"is_claimable":false,"name":"Portable sequencing of Mycobacterium tuberculosis for clinical and epidemiological applications.","description":"With >1 million associated deaths in 2020, human tuberculosis (TB) caused by the bacteria Mycobacterium tuberculosis remains one of the deadliest infectious diseases. A plethora of genomic tools and bioinformatics pipelines have become available in recent years to assist the whole genome sequencing of M. tuberculosis. The Oxford Nanopore Technologies (ONT) portable sequencer is a promising platform for cost-effective application in clinics, including personalizing treatment through detection of drug resistance-associated mutations, or in the field, to assist epidemiological and transmission investigations. In this study, we performed a comparison of 10 clinical isolates with DNA sequenced on both long-read ONT and (gold standard) short-read Illumina HiSeq platforms. Our analysis demonstrat","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2026-05-27T18:55:35.76Z","creation":"2025-02-19T04:38:12.971Z"},"accession":"S-EPMC9487601","cross_references":{"pubmed":["35894606"],"doi":["10.1093/bib/bbac256"]}}