biostudies-literatureGomez-Gonzalez PJNewton Institutional LinksMedical Research CouncilBiotechnology and Biological Sciences Research Councilbbac256https://www.ebi.ac.uk/biostudies/studies/S-EPMC9487601biostudies-literatureUnknown23(5)With >1 million associated deaths in 2020, human tuberculosis (TB) caused by the bacteria Mycobacterium tuberculosis remains one of the deadliest infectious diseases. A plethora of genomic tools and bioinformatics pipelines have become available in recent years to assist the whole genome sequencing of M. tuberculosis. The Oxford Nanopore Technologies (ONT) portable sequencer is a promising platform for cost-effective application in clinics, including personalizing treatment through detection of drug resistance-associated mutations, or in the field, to assist epidemiological and transmission investigations. In this study, we performed a comparison of 10 clinical isolates with DNA sequenced on both long-read ONT and (gold standard) short-read Illumina HiSeq platforms. Our analysis demonstratBriefings in bioinformaticsPortable sequencing of Mycobacterium tuberculosis for clinical and epidemiological applications.PMC9487601MR/N010469/1261868591MR/M01360X/1MR/R025576/1BB/R013063/1MR/R020973/1Campino SPhelan JEGomez-Gonzalez PJClark TGfalsePortable sequencing of Mycobacterium tuberculosis for clinical and epidemiological applications.With >1 million associated deaths in 2020, human tuberculosis (TB) caused by the bacteria Mycobacterium tuberculosis remains one of the deadliest infectious diseases. A plethora of genomic tools and bioinformatics pipelines have become available in recent years to assist the whole genome sequencing of M. tuberculosis. The Oxford Nanopore Technologies (ONT) portable sequencer is a promising platform for cost-effective application in clinics, including personalizing treatment through detection of drug resistance-associated mutations, or in the field, to assist epidemiological and transmission investigations. In this study, we performed a comparison of 10 clinical isolates with DNA sequenced on both long-read ONT and (gold standard) short-read Illumina HiSeq platforms. Our analysis demonstrat2022-01-01T00:00:00Z2022 Sep2026-05-27T18:55:35.76Z2025-02-19T04:38:12.971ZS-EPMC94876013589460610.1093/bib/bbac256