biostudies-literatureLi PFundamental Research Funds for the Central UniversitiesTsinghua UniversityBeijing Municipal Commission of Education100092https://www.ebi.ac.uk/biostudies/studies/S-EPMC9488089biostudies-literatureUnknown6In this study, an <i>Escherichia coli</i> (<i>E. coli</i>) whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein (GFP) reporter gene under the control of the ArsR1 (GSU2952) regulatory circuit from <i>Geobacter sulfurreducens</i>. <i>E. coli</i> cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium. Under optimal test conditions, the <i>Geobacter arsR1</i> promoter had a detection limit of 0.01 μM arsenite and the GFP expression was linear within a range of 0.03-0.1 μM (2.25-7.5 μg/l). These values were well below World Health Organization's drinking water quality standard, which is 10 μg/l. The feasibiliEnvironmental science and ecotechnologyDevelopment of a whole-cell biosensor based on an ArsR-P _<i>ars</i> regulatory circuit from <i>Geobacter sulfurreducens</i>.PMC9488089BLX2019342019ZY19Liu XFu XSun DLi PLiu CWang YYuan XDang YHolmes DEfalseDevelopment of a whole-cell biosensor based on an ArsR-P _<i>ars</i> regulatory circuit from <i>Geobacter sulfurreducens</i>.In this study, an <i>Escherichia coli</i> (<i>E. coli</i>) whole-cell biosensor for the specific detection of bioavailable arsenic was developed by placing a green fluorescent protein (GFP) reporter gene under the control of the ArsR1 (GSU2952) regulatory circuit from <i>Geobacter sulfurreducens</i>. <i>E. coli</i> cells only emitted green fluorescence in the presence of arsenite and were more sensitive to arsenite when they were grown in M9 supplemented medium compared to LB medium. Under optimal test conditions, the <i>Geobacter arsR1</i> promoter had a detection limit of 0.01 μM arsenite and the GFP expression was linear within a range of 0.03-0.1 μM (2.25-7.5 μg/l). These values were well below World Health Organization's drinking water quality standard, which is 10 μg/l. The feasibili2021-01-01T00:00:00Z2021 Apr2025-04-21T14:26:31.971Z2025-04-21T14:26:31.971ZS-EPMC94880893615918010.1016/j.ese.2021.100092