{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Goritzer K"],"funding":["European Research Council"],"pagination":["1003065"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9493077"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["Molecular pharming in plants offers exciting possibilities to address global access to modern biologics. However, differences in the <i>N</i>-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins. Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for <i>Arabidopsis thaliana</i> and <i>Nicotiana benthamiana</i>. Such engineering of <i>N</i>-glycosylation would also be desirable for <i>Nicotiana tabacum</i>, which remains the crop of choice for recombinant protein pharmaceuticals required at massive scale and for manufacturing technology transfer to less developed coun"],"journal":["Frontiers in plant science"],"pubmed_title":["Engineering the <i>N</i>-glycosylation pathway of <i>Nicotiana tabacum</i> for molecular pharming using CRISPR/Cas9."],"pmcid":["PMC9493077"],"funding_grant_id":["774078","760331"],"pubmed_authors":["Grandits M","Figl R","Ma JK","Navarre C","Teh AY","Goritzer K","Grunwald-Gruber C","Mercx S"],"additional_accession":[]},"is_claimable":false,"name":"Engineering the <i>N</i>-glycosylation pathway of <i>Nicotiana tabacum</i> for molecular pharming using CRISPR/Cas9.","description":"Molecular pharming in plants offers exciting possibilities to address global access to modern biologics. However, differences in the <i>N</i>-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins. Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for <i>Arabidopsis thaliana</i> and <i>Nicotiana benthamiana</i>. Such engineering of <i>N</i>-glycosylation would also be desirable for <i>Nicotiana tabacum</i>, which remains the crop of choice for recombinant protein pharmaceuticals required at massive scale and for manufacturing technology transfer to less developed coun","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-05T12:32:30.053Z","creation":"2025-04-05T12:32:30.053Z"},"accession":"S-EPMC9493077","cross_references":{"pubmed":["36161010"],"doi":["10.3389/fpls.2022.1003065"]}}