biostudies-literatureUnknown11(9)Akbar NWe examined the antiamoebic effect of several imidazothiazole derivatives on <i>Acanthamoeba castellanii</i> of the T4 genotype. Trypan blue exclusion assays and haemocytometer counting were used to determine the reduction in <i>A. castellanii</i> trophozoite proliferation, in response to treatment with these compounds. To determine the effects of these compounds on host cells, lactate dehydrogenase assay was performed using HeLa cell lines. Amoebicidal assays revealed that the tested compounds at concentrations of 50 µM significantly inhibited amoebae trophozoites compared to controls. Compounds <b>1m</b> and <b>1zb</b> showed the highest amoebicidal effects eradicating 70% and 67% of <i>A. castellanii</i>, respectively. The compounds blocked both the encystation and excystation process iAntibiotics (Basel, Switzerland)1183https://www.ebi.ac.uk/biostudies/studies/S-EPMC9494960biostudies-literatureAntiamoebic Activity of Imidazothiazole Derivatives against Opportunistic Pathogen <i>Acanthamoeba castellanii</i>.PMC9494960El-Gamal MIAlfahemi HOh CHSiddiqui RAkbar NSaeed BQKhan NAAbdel-Maksoud MSAlharbi AMfalseAntiamoebic Activity of Imidazothiazole Derivatives against Opportunistic Pathogen <i>Acanthamoeba castellanii</i>.We examined the antiamoebic effect of several imidazothiazole derivatives on <i>Acanthamoeba castellanii</i> of the T4 genotype. Trypan blue exclusion assays and haemocytometer counting were used to determine the reduction in <i>A. castellanii</i> trophozoite proliferation, in response to treatment with these compounds. To determine the effects of these compounds on host cells, lactate dehydrogenase assay was performed using HeLa cell lines. Amoebicidal assays revealed that the tested compounds at concentrations of 50 µM significantly inhibited amoebae trophozoites compared to controls. Compounds <b>1m</b> and <b>1zb</b> showed the highest amoebicidal effects eradicating 70% and 67% of <i>A. castellanii</i>, respectively. The compounds blocked both the encystation and excystation process i2022-01-01T00:00:00Z2022 Aug2025-04-25T19:14:02.697Z2025-04-06T07:51:32.664ZS-EPMC94949603613996210.3390/antibiotics11091183