{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Messelmani T"],"funding":["Agence Nationale de la Recherche"],"pagination":["443"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9495334"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(9)"],"pubmed_abstract":["The 3Rs guidelines recommend replacing animal testing with alternative models. One of the solutions proposed is organ-on-chip technology in which liver-on-chip is one of the most promising alternatives for drug screening and toxicological assays. The main challenge is to achieve the relevant in vivo-like functionalities of the liver tissue in an optimized cellular microenvironment. Here, we investigated the development of hepatic cells under dynamic conditions inside a 3D hydroscaffold embedded in a microfluidic device. The hydroscaffold is made of hyaluronic acid and composed of liver extracellular matrix components (galactosamine, collagen I/IV) with RGDS (Arg-Gly-Asp-Ser) sites for cell adhesion. The HepG2/C3A cell line was cultured under a flow rate of 10 µL/min for 21 days. After seed"],"journal":["Bioengineering (Basel, Switzerland)"],"pubmed_title":["Development of Liver-on-Chip Integrating a Hydroscaffold Mimicking the Liver's Extracellular Matrix."],"pmcid":["PMC9495334"],"funding_grant_id":["ANR-19-CE19-0020-01"],"pubmed_authors":["Jellali R","Le Goff A","Maes V","Messelmani T","Legallais C","Vandenhaute E","Roudaut M","Maubon N","Leclerc E","Souguir Z"],"additional_accession":[]},"is_claimable":false,"name":"Development of Liver-on-Chip Integrating a Hydroscaffold Mimicking the Liver's Extracellular Matrix.","description":"The 3Rs guidelines recommend replacing animal testing with alternative models. One of the solutions proposed is organ-on-chip technology in which liver-on-chip is one of the most promising alternatives for drug screening and toxicological assays. The main challenge is to achieve the relevant in vivo-like functionalities of the liver tissue in an optimized cellular microenvironment. Here, we investigated the development of hepatic cells under dynamic conditions inside a 3D hydroscaffold embedded in a microfluidic device. The hydroscaffold is made of hyaluronic acid and composed of liver extracellular matrix components (galactosamine, collagen I/IV) with RGDS (Arg-Gly-Asp-Ser) sites for cell adhesion. The HepG2/C3A cell line was cultured under a flow rate of 10 µL/min for 21 days. After seed","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-04T21:34:53.218Z","creation":"2025-04-04T21:34:53.218Z"},"accession":"S-EPMC9495334","cross_references":{"pubmed":["36134989"],"doi":["10.3390/bioengineering9090443"]}}