biostudies-literatureMesselmani TAgence Nationale de la Recherche443https://www.ebi.ac.uk/biostudies/studies/S-EPMC9495334biostudies-literatureUnknown9(9)The 3Rs guidelines recommend replacing animal testing with alternative models. One of the solutions proposed is organ-on-chip technology in which liver-on-chip is one of the most promising alternatives for drug screening and toxicological assays. The main challenge is to achieve the relevant in vivo-like functionalities of the liver tissue in an optimized cellular microenvironment. Here, we investigated the development of hepatic cells under dynamic conditions inside a 3D hydroscaffold embedded in a microfluidic device. The hydroscaffold is made of hyaluronic acid and composed of liver extracellular matrix components (galactosamine, collagen I/IV) with RGDS (Arg-Gly-Asp-Ser) sites for cell adhesion. The HepG2/C3A cell line was cultured under a flow rate of 10 µL/min for 21 days. After seedBioengineering (Basel, Switzerland)Development of Liver-on-Chip Integrating a Hydroscaffold Mimicking the Liver's Extracellular Matrix.PMC9495334ANR-19-CE19-0020-01Jellali RLe Goff AMaes VMesselmani TLegallais CVandenhaute ERoudaut MMaubon NLeclerc ESouguir ZfalseDevelopment of Liver-on-Chip Integrating a Hydroscaffold Mimicking the Liver's Extracellular Matrix.The 3Rs guidelines recommend replacing animal testing with alternative models. One of the solutions proposed is organ-on-chip technology in which liver-on-chip is one of the most promising alternatives for drug screening and toxicological assays. The main challenge is to achieve the relevant in vivo-like functionalities of the liver tissue in an optimized cellular microenvironment. Here, we investigated the development of hepatic cells under dynamic conditions inside a 3D hydroscaffold embedded in a microfluidic device. The hydroscaffold is made of hyaluronic acid and composed of liver extracellular matrix components (galactosamine, collagen I/IV) with RGDS (Arg-Gly-Asp-Ser) sites for cell adhesion. The HepG2/C3A cell line was cultured under a flow rate of 10 µL/min for 21 days. After seed2022-01-01T00:00:00Z2022 Sep2025-04-04T21:34:53.218Z2025-04-04T21:34:53.218ZS-EPMC94953343613498910.3390/bioengineering9090443