biostudies-literatureDu HNational Natural Science Foundation of China2725https://www.ebi.ac.uk/biostudies/studies/S-EPMC9498142biostudies-literatureUnknown11(18)<i>Pichia kudriavzevii</i> plays an important role in fermented foods and beverages. In the long domestication process of traditional fermentation, the mechanism of response to lactic acid, a common metabolite and growth inhibitor, is currently unclear in <i>P. kudriavzevii</i>. In this study, the tolerance to lactic acid of <i>P. kudriavzevii</i> C-16, isolated from fermented grains, was compared with its type strain ATCC 24210. Under lactic acid stress, <i>P. kudriavzevii</i> C-16 showed increased biomass yields and lactic acid consumption rates. Then, mRNA sequencing was used to analyze the response to lactic acid in <i>P. kudriavzevii</i> C-16. Results showed that 92 and 96 genes were significantly upregulated, 52 and 58 genes were significantly downregulated, respectively, in <i>P. kuFoods (Basel, Switzerland)Transcriptional Profiling Reveals Adaptive Response and Tolerance to Lactic Acid Stress in <i>Pichia kudriavzevii</i>.PMC949814232172176Deng NDu HFu YXu YfalseTranscriptional Profiling Reveals Adaptive Response and Tolerance to Lactic Acid Stress in <i>Pichia kudriavzevii</i>.<i>Pichia kudriavzevii</i> plays an important role in fermented foods and beverages. In the long domestication process of traditional fermentation, the mechanism of response to lactic acid, a common metabolite and growth inhibitor, is currently unclear in <i>P. kudriavzevii</i>. In this study, the tolerance to lactic acid of <i>P. kudriavzevii</i> C-16, isolated from fermented grains, was compared with its type strain ATCC 24210. Under lactic acid stress, <i>P. kudriavzevii</i> C-16 showed increased biomass yields and lactic acid consumption rates. Then, mRNA sequencing was used to analyze the response to lactic acid in <i>P. kudriavzevii</i> C-16. Results showed that 92 and 96 genes were significantly upregulated, 52 and 58 genes were significantly downregulated, respectively, in <i>P. ku2022-01-01T00:00:00Z2022 Sep2025-04-22T04:07:54.014Z2025-04-05T20:55:52.791ZS-EPMC94981423614085410.3390/foods11182725