{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bennett NK"],"funding":["University of California Cancer Research Coordinating Committee","NIA NIH HHS","NCI NIH HHS","NINDS NIH HHS","National Institutes of Health","Joan and David Traitel Family Trust","Japan Society for the Promotion of Science"],"pagination":["e3001753"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9498964"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(9)"],"pubmed_abstract":["The Warburg effect, aerobic glycolysis, is a hallmark feature of cancer cells grown in culture. However, the relative roles of glycolysis and respiratory metabolism in supporting in vivo tumor growth and processes such as tumor dissemination and metastatic growth remain poorly understood, particularly on a systems level. Using a CRISPRi mini-library enriched for mitochondrial ribosomal protein and respiratory chain genes in multiple human lung cancer cell lines, we analyzed in vivo metabolic requirements in xenograft tumors grown in distinct anatomic contexts. While knockdown of mitochondrial ribosomal protein and respiratory chain genes (mito-respiratory genes) has little impact on growth in vitro, tumor cells depend heavily on these genes when grown in vivo as either flank or primary ort"],"journal":["PLoS biology"],"pubmed_title":["Primary and metastatic tumors exhibit systems-level differences in dependence on mitochondrial respiratory function."],"pmcid":["PMC9498964"],"funding_grant_id":["U54 CA196519","R01AG065428","RO1NS091902","R01 AG065428","U54CA196519","P50 CA211015","K08 CA222625","R37 CA255453","R01 NS091902"],"pubmed_authors":["Sei Y","Nakamura JL","Okimoto RA","Bivona TG","Bennett NK","Nakaoka HJ","Nakamura K","Ten Hoeve J","Laurent D","Horvai AE","Graeber TG"],"additional_accession":[]},"is_claimable":false,"name":"Primary and metastatic tumors exhibit systems-level differences in dependence on mitochondrial respiratory function.","description":"The Warburg effect, aerobic glycolysis, is a hallmark feature of cancer cells grown in culture. However, the relative roles of glycolysis and respiratory metabolism in supporting in vivo tumor growth and processes such as tumor dissemination and metastatic growth remain poorly understood, particularly on a systems level. Using a CRISPRi mini-library enriched for mitochondrial ribosomal protein and respiratory chain genes in multiple human lung cancer cell lines, we analyzed in vivo metabolic requirements in xenograft tumors grown in distinct anatomic contexts. While knockdown of mitochondrial ribosomal protein and respiratory chain genes (mito-respiratory genes) has little impact on growth in vitro, tumor cells depend heavily on these genes when grown in vivo as either flank or primary ort","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-04T09:30:27.611Z","creation":"2025-02-19T00:42:05.075Z"},"accession":"S-EPMC9498964","cross_references":{"pubmed":["36137002"],"doi":["10.1371/journal.pbio.3001753"]}}