biostudies-literatureLogan RAEEconomic and Social Research CouncilBrock UniversityGouvernement du Canada | Natural Sciences and Engineering Research Council of CanadaUKRI | Biotechnology and Biological Sciences Research CouncilUKRI | Engineering and Physical Sciences Research CouncilNational Institute for Health Research (NIHR)Liverpool School of Tropical MedicineNational Institute for Health and Care ResearchRoyal SocietyWellcome TrustUK Research and InnovationBiotechnology and Biological Sciences Research Councile0106222https://www.ebi.ac.uk/biostudies/studies/S-EPMC9499017biostudies-literatureUnknown88(18)Cell fusing agent virus (CFAV) is an insect-specific flavivirus (ISF) found in Aedes aegypti mosquitoes. ISFs have demonstrated the ability to modulate the infection or transmission of arboviruses such as dengue, West Nile, and Zika viruses. It is thought that vertical transmission is the main route for ISF maintenance in nature. This has been observed with CFAV, but there is evidence of horizontal and venereal transmission in other ISFs. Understanding the route of transmission can inform strategies to spread ISFs to vector populations as a method of controlling pathogenic arboviruses. We crossed individually reared male and female mosquitoes from both a naturally occurring CFAV-positive Ae. aegypti colony and its negative counterpart to provide information on maternal, paternal, and horizApplied and environmental microbiologyVertical and Horizontal Transmission of Cell Fusing Agent Virus in Aedes aegypti.PMC9499017RGPIN-2022-05099BB/V011278/1SIPF00005/1RSWF\R1\180013V011278/1219672/Z/19/Z20197BB/W018446/1NIHR2000907BB/T001240/1V043811/1Muthoni JNQuek SPatterson GTHeinz EVillena MENHegde Svon Eicken ABrettell LEAnderson ERLogan RAEPatterson EIHughes GLfalseVertical and Horizontal Transmission of Cell Fusing Agent Virus in Aedes aegypti.Cell fusing agent virus (CFAV) is an insect-specific flavivirus (ISF) found in Aedes aegypti mosquitoes. ISFs have demonstrated the ability to modulate the infection or transmission of arboviruses such as dengue, West Nile, and Zika viruses. It is thought that vertical transmission is the main route for ISF maintenance in nature. This has been observed with CFAV, but there is evidence of horizontal and venereal transmission in other ISFs. Understanding the route of transmission can inform strategies to spread ISFs to vector populations as a method of controlling pathogenic arboviruses. We crossed individually reared male and female mosquitoes from both a naturally occurring CFAV-positive Ae. aegypti colony and its negative counterpart to provide information on maternal, paternal, and horiz2022-01-01T00:00:00Z2022 Sep2025-04-05T11:43:20.754Z2025-02-18T22:34:25.134ZS-EPMC94990173603657710.1128/aem.01062-22