biostudies-literatureJiang HShaanxi Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of China10445https://www.ebi.ac.uk/biostudies/studies/S-EPMC9499528biostudies-literatureUnknown23(18)Fusarium head blight (FHB), caused by the fungal pathogen <i>Fusarium graminearum</i>, is a destructive disease worldwide. Ascospores are the primary inoculum of <i>F. graminearum</i>, and sexual reproduction is a critical step in its infection cycle. In this study, we characterized the functions of FgCsn12. Although the ortholog of FgCsn12 in budding yeast was reported to have a direct interaction with Csn5, which served as the core subunit of the COP9 signalosome, the interaction between FgCsn12 and FgCsn5 was not detected through the yeast two-hybrid assay. The deletion of <i>FgCSN12</i> resulted in slight defects in the growth rate, conidial morphology, and pathogenicity. Instead of forming four-celled, uninucleate ascospores, the <i>Fgcsn12</i> deletion mutant produced oval ascosporesInternational journal of molecular sciencesFgCsn12 Is Involved in the Regulation of Ascosporogenesis in the Wheat Scab Fungus <i>Fusarium graminearum</i>.PMC94995282022JC-1432102181Liu HCao XQi JZhang YWang CJiang HWang WJiang CXu HfalseFgCsn12 Is Involved in the Regulation of Ascosporogenesis in the Wheat Scab Fungus <i>Fusarium graminearum</i>.Fusarium head blight (FHB), caused by the fungal pathogen <i>Fusarium graminearum</i>, is a destructive disease worldwide. Ascospores are the primary inoculum of <i>F. graminearum</i>, and sexual reproduction is a critical step in its infection cycle. In this study, we characterized the functions of FgCsn12. Although the ortholog of FgCsn12 in budding yeast was reported to have a direct interaction with Csn5, which served as the core subunit of the COP9 signalosome, the interaction between FgCsn12 and FgCsn5 was not detected through the yeast two-hybrid assay. The deletion of <i>FgCSN12</i> resulted in slight defects in the growth rate, conidial morphology, and pathogenicity. Instead of forming four-celled, uninucleate ascospores, the <i>Fgcsn12</i> deletion mutant produced oval ascospores2022-01-01T00:00:00Z2022 Sep2025-04-21T16:10:00.472Z2025-02-19T00:42:06.432ZS-EPMC94995283614235610.3390/ijms231810445