{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wallace RL"],"funding":["University of Arizona","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["e68773"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9499541"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11"],"pubmed_abstract":["The target of rapamycin complex I (TORC1) regulates cell growth and metabolism in eukaryotes. Previous studies have shown that nitrogen and amino acid signals activate TORC1 via the highly conserved small GTPases, Gtr1/2 (RagA/C in humans), and the GTPase activating complex SEAC/GATOR. However, it remains unclear if, and how, other proteins/pathways regulate TORC1 in simple eukaryotes like yeast. Here, we report that the previously unstudied GPCR-like protein, Ait1, binds to TORC1-Gtr1/2 in <i>Saccharomyces cerevisiae</i> and holds TORC1 around the vacuole during log-phase growth. Then, during amino acid starvation, Ait1 inhibits TORC1 via Gtr1/2 using a loop that resembles the RagA/C-binding domain in the human protein SLC38A9. Importantly, Ait1 is only found in the <i>Saccharomycetaceae/"],"journal":["eLife"],"pubmed_title":["Ait1 regulates TORC1 signaling and localization in budding yeast."],"pmcid":["PMC9499541"],"funding_grant_id":["R01 GM097329","R01GM097329","T32GM136536","T32 GM136536"],"pubmed_authors":["Wallace RL","Lu E","Luo X","Capaldi AP"],"additional_accession":[]},"is_claimable":false,"name":"Ait1 regulates TORC1 signaling and localization in budding yeast.","description":"The target of rapamycin complex I (TORC1) regulates cell growth and metabolism in eukaryotes. Previous studies have shown that nitrogen and amino acid signals activate TORC1 via the highly conserved small GTPases, Gtr1/2 (RagA/C in humans), and the GTPase activating complex SEAC/GATOR. However, it remains unclear if, and how, other proteins/pathways regulate TORC1 in simple eukaryotes like yeast. Here, we report that the previously unstudied GPCR-like protein, Ait1, binds to TORC1-Gtr1/2 in <i>Saccharomyces cerevisiae</i> and holds TORC1 around the vacuole during log-phase growth. Then, during amino acid starvation, Ait1 inhibits TORC1 via Gtr1/2 using a loop that resembles the RagA/C-binding domain in the human protein SLC38A9. Importantly, Ait1 is only found in the <i>Saccharomycetaceae/","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-26T22:06:51.606Z","creation":"2025-04-06T17:04:25.135Z"},"accession":"S-EPMC9499541","cross_references":{"pubmed":["36047762"],"doi":["10.7554/eLife.68773"]}}