{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Idiago-Lopez J"],"funding":["Ministerio de Universidades","Ministerio de Asuntos Econ?micos y Transformaci?n Digital, Gobierno de Espa?a","Gobierno de Arag?n","Ministerio de Ciencia, Innovaci?n y Universidades","Eusko Jaurlaritza","Agencia Estatal de Investigaci?n","European Commission","Ministerio de Econom?a y Competitividad","Fondos Feder","Operativo Fondo Social Europeo de  Arag?n"],"pagination":["1620-1633"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9501912"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(9)"],"pubmed_abstract":["In this work, we report the use of bioorthogonal chemistry, specifically the strain-promoted click azide-alkyne cycloaddition (SPAAC) for the covalent attachment of magnetic nanoparticles (MNPs) on living cell membranes. Four types of MNPs were prepared, functionalized with two different stabilizing/passivation agents (a polyethylene glycol derivative and a glucopyranoside derivative, respectively) and two types of strained alkynes with different reactivities: a cyclooctyne (CO) derivative and a dibenzocyclooctyne (DBCO) derivative. The MNPs were extensively characterized in terms of physicochemical characteristics, colloidal stability, and click reactivity in suspension. Then, the reactivity of the MNPs toward azide-modified surfaces was evaluated as a closer approach to their final appli"],"journal":["Bioconjugate chemistry"],"pubmed_title":["From Bench to Cell: A Roadmap for Assessing the Bioorthogonal \"Click\" Reactivity of Magnetic Nanoparticles for Cell Surface Engineering."],"pmcid":["PMC9501912"],"funding_grant_id":["DGA 2017-2021","RYC-2015-17640","FPU17/02024","PCIN-2017-060","PGC2018-096016-B-I00","GIC-2015_IT-1033-16","E15_20R","BIO 2017-84246-C2-1R"],"pubmed_authors":["Eceiza M","Fratila RM","Grazu V","Idiago-Lopez J","Moreno-Antolin E","de la Fuente JM","Aizpurua JM"],"additional_accession":[]},"is_claimable":false,"name":"From Bench to Cell: A Roadmap for Assessing the Bioorthogonal \"Click\" Reactivity of Magnetic Nanoparticles for Cell Surface Engineering.","description":"In this work, we report the use of bioorthogonal chemistry, specifically the strain-promoted click azide-alkyne cycloaddition (SPAAC) for the covalent attachment of magnetic nanoparticles (MNPs) on living cell membranes. Four types of MNPs were prepared, functionalized with two different stabilizing/passivation agents (a polyethylene glycol derivative and a glucopyranoside derivative, respectively) and two types of strained alkynes with different reactivities: a cyclooctyne (CO) derivative and a dibenzocyclooctyne (DBCO) derivative. The MNPs were extensively characterized in terms of physicochemical characteristics, colloidal stability, and click reactivity in suspension. Then, the reactivity of the MNPs toward azide-modified surfaces was evaluated as a closer approach to their final appli","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-19T14:23:13.831Z","creation":"2024-11-12T22:01:37.427Z"},"accession":"S-EPMC9501912","cross_references":{"pubmed":["35857350"],"doi":["10.1021/acs.bioconjchem.2c00230"]}}