biostudies-literatureIdiago-Lopez JMinisterio de UniversidadesMinisterio de Asuntos Econ?micos y Transformaci?n Digital, Gobierno de Espa?aGobierno de Arag?nMinisterio de Ciencia, Innovaci?n y UniversidadesEusko JaurlaritzaAgencia Estatal de Investigaci?nEuropean CommissionMinisterio de Econom?a y CompetitividadFondos FederOperativo Fondo Social Europeo de Arag?n1620-1633https://www.ebi.ac.uk/biostudies/studies/S-EPMC9501912biostudies-literatureUnknown33(9)In this work, we report the use of bioorthogonal chemistry, specifically the strain-promoted click azide-alkyne cycloaddition (SPAAC) for the covalent attachment of magnetic nanoparticles (MNPs) on living cell membranes. Four types of MNPs were prepared, functionalized with two different stabilizing/passivation agents (a polyethylene glycol derivative and a glucopyranoside derivative, respectively) and two types of strained alkynes with different reactivities: a cyclooctyne (CO) derivative and a dibenzocyclooctyne (DBCO) derivative. The MNPs were extensively characterized in terms of physicochemical characteristics, colloidal stability, and click reactivity in suspension. Then, the reactivity of the MNPs toward azide-modified surfaces was evaluated as a closer approach to their final appliBioconjugate chemistryFrom Bench to Cell: A Roadmap for Assessing the Bioorthogonal "Click" Reactivity of Magnetic Nanoparticles for Cell Surface Engineering.PMC9501912DGA 2017-2021RYC-2015-17640FPU17/02024PCIN-2017-060PGC2018-096016-B-I00GIC-2015_IT-1033-16E15_20RBIO 2017-84246-C2-1REceiza MFratila RMGrazu VIdiago-Lopez JMoreno-Antolin Ede la Fuente JMAizpurua JMfalseFrom Bench to Cell: A Roadmap for Assessing the Bioorthogonal "Click" Reactivity of Magnetic Nanoparticles for Cell Surface Engineering.In this work, we report the use of bioorthogonal chemistry, specifically the strain-promoted click azide-alkyne cycloaddition (SPAAC) for the covalent attachment of magnetic nanoparticles (MNPs) on living cell membranes. Four types of MNPs were prepared, functionalized with two different stabilizing/passivation agents (a polyethylene glycol derivative and a glucopyranoside derivative, respectively) and two types of strained alkynes with different reactivities: a cyclooctyne (CO) derivative and a dibenzocyclooctyne (DBCO) derivative. The MNPs were extensively characterized in terms of physicochemical characteristics, colloidal stability, and click reactivity in suspension. Then, the reactivity of the MNPs toward azide-modified surfaces was evaluated as a closer approach to their final appli2022-01-01T00:00:00Z2022 Sep2025-04-19T14:23:13.831Z2024-11-12T22:01:37.427ZS-EPMC95019123585735010.1021/acs.bioconjchem.2c00230