{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shu M"],"funding":["Changsha key S&amp;amp;T Special Projects","Education Department of Scientific Research Project of Hunan Province","Natural Science Foundation of Hunan Province","Research and Development Plan in Key Areas of Hunan Province","National Natural Science Foundation of China","Education Department of Postgraduate Research and Innovation Project of Hunan Province","Changsha key S&amp;T Special Projects","the Research and Development Plan in Key Areas of Hunan Province"],"pagination":["910"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9503372"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(9)"],"pubmed_abstract":["In this study, the gene <i>monascus</i>-5162 from <i>Monascus purpureus</i> LQ-6, identified as chitin synthase gene VI (<i>chs</i>6), was knocked out to disrupt the chitin biosynthetic pathway and regulate the biosynthesis of <i>Monascus</i> pigments (MPs) and citrinin. The results showed that the aerial hyphae on a solid medium were short and sparse after the deletion of <i>chs</i>6 in <i>M. purpureus</i> LQ-6, significantly reducing the germination percentage of active spores to approximately 22%, but the colony diameter was almost unaffected. Additionally, the deletion of <i>chs</i>6 changed the mycelial morphology of <i>M. purpureus</i> LQ-6 during submerged fermentation and increased its sensitivity to environmental factors. MP and citrinin biosynthesis was dramatically inhibited in "],"journal":["Journal of fungi (Basel, Switzerland)"],"pubmed_title":["Disruption of the Chitin Biosynthetic Pathway Results in Significant Changes in the Cell Growth Phenotypes and Biosynthesis of Secondary Metabolites of <i>Monascus purpureus</i>."],"pmcid":["PMC9503372"],"funding_grant_id":["32101906","kh2003013","No. 32101906","No. 2021JJ31146, No. 2022JJ31011","No. kh2003013","20B619","No. 2019NK2111","2022JJ31011","CX20210863","2021JJ31146","No. CX20210898, No. CX20210863","CX20210898","2019NK2111"],"pubmed_authors":["Lin Q","Liu J","Zhou B","Liu S","Zhang S","Gong Z","Lu P","Cai X","Shu M"],"additional_accession":[]},"is_claimable":false,"name":"Disruption of the Chitin Biosynthetic Pathway Results in Significant Changes in the Cell Growth Phenotypes and Biosynthesis of Secondary Metabolites of <i>Monascus purpureus</i>.","description":"In this study, the gene <i>monascus</i>-5162 from <i>Monascus purpureus</i> LQ-6, identified as chitin synthase gene VI (<i>chs</i>6), was knocked out to disrupt the chitin biosynthetic pathway and regulate the biosynthesis of <i>Monascus</i> pigments (MPs) and citrinin. The results showed that the aerial hyphae on a solid medium were short and sparse after the deletion of <i>chs</i>6 in <i>M. purpureus</i> LQ-6, significantly reducing the germination percentage of active spores to approximately 22%, but the colony diameter was almost unaffected. Additionally, the deletion of <i>chs</i>6 changed the mycelial morphology of <i>M. purpureus</i> LQ-6 during submerged fermentation and increased its sensitivity to environmental factors. MP and citrinin biosynthesis was dramatically inhibited in ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2026-04-08T11:48:54.74Z","creation":"2024-12-03T20:18:45.915Z"},"accession":"S-EPMC9503372","cross_references":{"pubmed":["36135635"],"doi":["10.3390/jof8090910"]}}