biostudies-literatureShu MChangsha key S&amp;amp;T Special ProjectsEducation Department of Scientific Research Project of Hunan ProvinceNatural Science Foundation of Hunan ProvinceResearch and Development Plan in Key Areas of Hunan ProvinceNational Natural Science Foundation of ChinaEducation Department of Postgraduate Research and Innovation Project of Hunan ProvinceChangsha key S&amp;T Special Projectsthe Research and Development Plan in Key Areas of Hunan Province910https://www.ebi.ac.uk/biostudies/studies/S-EPMC9503372biostudies-literatureUnknown8(9)In this study, the gene <i>monascus</i>-5162 from <i>Monascus purpureus</i> LQ-6, identified as chitin synthase gene VI (<i>chs</i>6), was knocked out to disrupt the chitin biosynthetic pathway and regulate the biosynthesis of <i>Monascus</i> pigments (MPs) and citrinin. The results showed that the aerial hyphae on a solid medium were short and sparse after the deletion of <i>chs</i>6 in <i>M. purpureus</i> LQ-6, significantly reducing the germination percentage of active spores to approximately 22%, but the colony diameter was almost unaffected. Additionally, the deletion of <i>chs</i>6 changed the mycelial morphology of <i>M. purpureus</i> LQ-6 during submerged fermentation and increased its sensitivity to environmental factors. MP and citrinin biosynthesis was dramatically inhibited in Journal of fungi (Basel, Switzerland)Disruption of the Chitin Biosynthetic Pathway Results in Significant Changes in the Cell Growth Phenotypes and Biosynthesis of Secondary Metabolites of <i>Monascus purpureus</i>.PMC950337232101906kh2003013No. 32101906No. 2021JJ31146, No. 2022JJ31011No. kh200301320B619No. 2019NK21112022JJ31011CX202108632021JJ31146No. CX20210898, No. CX20210863CX202108982019NK2111Lin QLiu JZhou BLiu SZhang SGong ZLu PCai XShu MfalseDisruption of the Chitin Biosynthetic Pathway Results in Significant Changes in the Cell Growth Phenotypes and Biosynthesis of Secondary Metabolites of <i>Monascus purpureus</i>.In this study, the gene <i>monascus</i>-5162 from <i>Monascus purpureus</i> LQ-6, identified as chitin synthase gene VI (<i>chs</i>6), was knocked out to disrupt the chitin biosynthetic pathway and regulate the biosynthesis of <i>Monascus</i> pigments (MPs) and citrinin. The results showed that the aerial hyphae on a solid medium were short and sparse after the deletion of <i>chs</i>6 in <i>M. purpureus</i> LQ-6, significantly reducing the germination percentage of active spores to approximately 22%, but the colony diameter was almost unaffected. Additionally, the deletion of <i>chs</i>6 changed the mycelial morphology of <i>M. purpureus</i> LQ-6 during submerged fermentation and increased its sensitivity to environmental factors. MP and citrinin biosynthesis was dramatically inhibited in 2022-01-01T00:00:00Z2022 Aug2026-04-08T11:48:54.74Z2024-12-03T20:18:45.915ZS-EPMC95033723613563510.3390/jof8090910