biostudies-literatureUnknown11(18)Nogbou NDCarbapenemase-producing <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>) is resistant to most of the available antibiotics and poses serious therapeutic challenges. The study investigated <i>Monsonia angustifolia</i> (<i>M. angustifolia</i>) and <i>Momordica balsamina Linn</i> (<i>M. balsamina Linn</i>) extracts for antibacterial activity against a clinical isolate of carbapenemase-producing <i>A. baumannii</i> using the Kirby Bauer disc diffusion and TLC coupled with bioautography. MIC determination experiments were conducted on a molecularly characterized <i>A. baumannii</i> isolate identified using VITEK2. Positive PCR detection of <i>bla_OXA-51</i> and <i>bla_OXA-23</i> confirmed isolate identity and the presence of a carbapenemase-encoding gene. Antibacterial activity was observed with the methanolic extract of <i>M. balsamina Linn</i> with a MIC of 0.5 mg/mL. Compounds with Rf values of 0.05; 0.17; 0.39 obtained from <i>M. angustifolia</i> hexane extract; compounds with Rf values of 0.58; 0.78; 0.36; 0.48; 0.5; 0.56; 0.67; 0.9 obtained from <i>M. angustifolia</i> dichloromethane extract; compounds with Rf values of 0.11; 0.56; 0.24; 0.37 obtained from <i>M. angustifolia</i> acetone extract and compounds with Rf values of 0.11; 0.27 obtained from <i>M. angustifolia</i> methanol extract demonstrated a level of antibacterial activity. <i>M. angustifolia</i> and <i>M. balsamina Linn</i> plant extracts have a clinically significant antibacterial activity against a carbapenemase-producing <i>A. baumannii</i> strain.Plants (Basel, Switzerland)2374https://www.ebi.ac.uk/biostudies/studies/S-EPMC9503486biostudies-literatureAntibacterial Activities of Monsonia Angustifolia and Momordica Balsamina Linn Extracts against Carbapenem-Resistant Acinetobacter Baumannii.PMC9503486Mabela DRMothibe MEObi LCMatseke BMapfumari NSMusyoki AMNogbou NDfalseAntibacterial Activities of Monsonia Angustifolia and Momordica Balsamina Linn Extracts against Carbapenem-Resistant Acinetobacter Baumannii.Carbapenemase-producing <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>) is resistant to most of the available antibiotics and poses serious therapeutic challenges. The study investigated <i>Monsonia angustifolia</i> (<i>M. angustifolia</i>) and <i>Momordica balsamina Linn</i> (<i>M. balsamina Linn</i>) extracts for antibacterial activity against a clinical isolate of carbapenemase-producing <i>A. baumannii</i> using the Kirby Bauer disc diffusion and TLC coupled with bioautography. MIC determination experiments were conducted on a molecularly characterized <i>A. baumannii</i> isolate identified using VITEK2. Positive PCR detection of <i>bla_OXA-51</i> and <i>bla_OXA-23</i> confirmed isolate identity and the presence of a carbapenemase-encoding gene. Antibacterial activity was observed with the methanolic extract of <i>M. balsamina Linn</i> with a MIC of 0.5 mg/mL. Compounds with Rf values of 0.05; 0.17; 0.39 obtained from <i>M. angustifolia</i> hexane extract; compounds with Rf values of 0.58; 0.78; 0.36; 0.48; 0.5; 0.56; 0.67; 0.9 obtained from <i>M. angustifolia</i> dichloromethane extract; compounds with Rf values of 0.11; 0.56; 0.24; 0.37 obtained from <i>M. angustifolia</i> acetone extract and compounds with Rf values of 0.11; 0.27 obtained from <i>M. angustifolia</i> methanol extract demonstrated a level of antibacterial activity. <i>M. angustifolia</i> and <i>M. balsamina Linn</i> plant extracts have a clinically significant antibacterial activity against a carbapenemase-producing <i>A. baumannii</i> strain.2022-01-01T00:00:00Z2022 Sep2025-04-22T05:40:51.019Z2025-02-19T00:41:59.686ZS-EPMC95034863614577610.3390/plants11182374