biostudies-literatureLi SGuizhou Province Science and Technology ProjectNational Key Research and Development Program of China10484https://www.ebi.ac.uk/biostudies/studies/S-EPMC9503964biostudies-literatureUnknown23(18)Sclerotia of <i>Wolfiporia hoelen</i> are one of the most important traditional Chinese medicines and are commonly used in China, Japan, Korea, and other Asian countries. In the present study, we presented the first high-quality homokaryotic genome of <i>W. hoelen</i> with 14 chromosomes which was evaluated with assembly index, telomere position detection, and whole-genome collinearity. A 64.44 Mb genome was assembled with a Contig N50 length of 3.76 Mb. The imbalanced distribution of transposons and chromosome characters revealed the probable two-speed genome of <i>W. hoelen</i>. High consistency between methylation and transposon conserved the genome stability. The expansion of the gene family about signal transduction and nutritional transport has intimate relationships with sclerotial International journal of molecular sciencesHomokaryotic High-Quality Genome Assembly of Medicinal Fungi <i>Wolfiporia hoelen</i> Reveals Auto-Regulation and High-Temperature Adaption of Probable Two-Speed Genome.PMC95039642017YFC1703003QKHZC[2021]249Li SDong CMeng GfalseHomokaryotic High-Quality Genome Assembly of Medicinal Fungi <i>Wolfiporia hoelen</i> Reveals Auto-Regulation and High-Temperature Adaption of Probable Two-Speed Genome.Sclerotia of <i>Wolfiporia hoelen</i> are one of the most important traditional Chinese medicines and are commonly used in China, Japan, Korea, and other Asian countries. In the present study, we presented the first high-quality homokaryotic genome of <i>W. hoelen</i> with 14 chromosomes which was evaluated with assembly index, telomere position detection, and whole-genome collinearity. A 64.44 Mb genome was assembled with a Contig N50 length of 3.76 Mb. The imbalanced distribution of transposons and chromosome characters revealed the probable two-speed genome of <i>W. hoelen</i>. High consistency between methylation and transposon conserved the genome stability. The expansion of the gene family about signal transduction and nutritional transport has intimate relationships with sclerotial 2022-01-01T00:00:00Z2022 Sep2025-05-18T12:49:42.594Z2025-05-18T12:49:42.594ZS-EPMC95039643614239710.3390/ijms231810484