biostudies-literatureUnknown10(9)Samsonov VVIn <i>E. coli</i> and <i>P. ananatis</i>, L-serine biosynthesis is initiated by the action of D-3-phosphoglycerate dehydrogenase (SerA), which converts D-3-phosphoglycerate into 3-phosphohydroxypyruvate. SerA can concomitantly catalyze the production of D-2-hydroxyglutarate (D-2-HGA) from 2-ketoglutarate by oxidizing NADH to NAD⁺. Several bacterial D-2-hydroxyglutarate dehydrogenases (D2HGDHs) have recently been identified, which convert D-2-HGA back to 2-ketoglutarate. However, knowledge about the enzymes that can metabolize D-2-HGA is lacking in bacteria belonging to the <i>Enterobacteriaceae</i> family. We found that <i>ydiJ</i> encodes novel D2HGDHs in <i>P. ananatis</i> and <i>E. coli</i>, which were assigned as D2HGDH<i>Pa</i> and D2HGDH<i>Ec</i>, respectively. InactivatioMicroorganisms1766https://www.ebi.ac.uk/biostudies/studies/S-EPMC9504171biostudies-literatureRevealing a New Family of D-2-Hydroxyglutarate Dehydrogenases in <i>Escherichia coli</i> and <i>Pantoea ananatis</i> Encoded by <i>ydiJ</i>.PMC9504171Rostova JGKuznetsova AASamsonov VVZiyatdinov MKSamsonova SAKiriukhin MYfalseRevealing a New Family of D-2-Hydroxyglutarate Dehydrogenases in <i>Escherichia coli</i> and <i>Pantoea ananatis</i> Encoded by <i>ydiJ</i>.In <i>E. coli</i> and <i>P. ananatis</i>, L-serine biosynthesis is initiated by the action of D-3-phosphoglycerate dehydrogenase (SerA), which converts D-3-phosphoglycerate into 3-phosphohydroxypyruvate. SerA can concomitantly catalyze the production of D-2-hydroxyglutarate (D-2-HGA) from 2-ketoglutarate by oxidizing NADH to NAD⁺. Several bacterial D-2-hydroxyglutarate dehydrogenases (D2HGDHs) have recently been identified, which convert D-2-HGA back to 2-ketoglutarate. However, knowledge about the enzymes that can metabolize D-2-HGA is lacking in bacteria belonging to the <i>Enterobacteriaceae</i> family. We found that <i>ydiJ</i> encodes novel D2HGDHs in <i>P. ananatis</i> and <i>E. coli</i>, which were assigned as D2HGDH<i>Pa</i> and D2HGDH<i>Ec</i>, respectively. Inactivatio2022-01-01T00:00:00Z2022 Aug2026-04-08T10:45:51.097Z2024-12-04T01:39:32.861ZS-EPMC95041713614436810.3390/microorganisms10091766