biostudies-literatureHandlovic TTNational Cancer InstituteNCI NIH HHSNational Institutes of HealthWelch FoundationNational Science Foundation340156https://www.ebi.ac.uk/biostudies/studies/S-EPMC9504283biostudies-literatureUnknown1228Sub/supercritical fluid chromatography (SFC) is a green separation technique that has been used to separate a wide variety of compounds and is proven to be immensely useful for chiral separations. However, SFC is currently not thought to be applicable for ionic compounds due to their low solubility in CO₂, even with additives and organic modifiers. Recently, a large amount of research has been centered on octahedral complexes of Ru(II) and Os(II) with bidentate polypyridyl ligands due to their ability to serve in cancer treatment and other biological activities. These compounds exist as the delta (Δ) and lambda (Λ) enantiomers. Previously, similar compounds have been enantiomerically separated using HPLC and capillary electrophoresis, but never with SFC. Cyclofructan-6 (CF6) derAnalytica chimica actaInsights into enantioselective separations of ionic metal complexes by sub/supercritical fluid chromatography.PMC9504283R01 CA2222272102459Y-0026R01CA222227Wahab MFHandlovic TTMcFarland SARamasamy EArmstrong DWCole HDAlatrash NMacDonnell FMfalseInsights into enantioselective separations of ionic metal complexes by sub/supercritical fluid chromatography.Sub/supercritical fluid chromatography (SFC) is a green separation technique that has been used to separate a wide variety of compounds and is proven to be immensely useful for chiral separations. However, SFC is currently not thought to be applicable for ionic compounds due to their low solubility in CO₂, even with additives and organic modifiers. Recently, a large amount of research has been centered on octahedral complexes of Ru(II) and Os(II) with bidentate polypyridyl ligands due to their ability to serve in cancer treatment and other biological activities. These compounds exist as the delta (Δ) and lambda (Λ) enantiomers. Previously, similar compounds have been enantiomerically separated using HPLC and capillary electrophoresis, but never with SFC. Cyclofructan-6 (CF6) der2022-01-01T00:00:00Z2022 Oct2025-04-18T22:45:52.614Z2025-04-07T10:24:27.618ZS-EPMC95042833612699810.1016/j.aca.2022.340156