biostudies-literatureWiley MBNIDDK NIH HHSCenter for Medicinal Cannabis ResearchNational Institute of Diabetes and Digestive Kidney DiseasesCenter for Medicinal Cannabis Research (CMCR) at the University of California-San Diego10549https://www.ebi.ac.uk/biostudies/studies/S-EPMC9504303biostudies-literatureUnknown23(18)The gut barrier provides protection from pathogens and its function is compromised in diet-induced obesity (DIO). The endocannabinoid system in the gut is dysregulated in DIO and participates in gut barrier function; however, whether its activity is protective or detrimental for gut barrier integrity is unclear. We used mice conditionally deficient in cannabinoid receptor subtype-1 (CB₁R) in the intestinal epithelium (intCB1-/-) to test the hypothesis that CB₁Rs in intestinal epithelial cells provide protection from diet-induced gut barrier dysfunction. Control and intCB1-/- mice were placed for eight weeks on a high-fat/sucrose Western-style diet (WD) or a low-fat/no-sucrose diet. Endocannabinoid levels and activity of their metabolic enzymes were measured in the larInternational journal of molecular sciencesDiet-Induced Gut Barrier Dysfunction Is Exacerbated in Mice Lacking Cannabinoid 1 Receptors in the Intestinal Epithelium.PMC9504303874959R01DK119498Pilot Project 874959R01 DK119498Wiley MBDiPatrizio NVfalseDiet-Induced Gut Barrier Dysfunction Is Exacerbated in Mice Lacking Cannabinoid 1 Receptors in the Intestinal Epithelium.The gut barrier provides protection from pathogens and its function is compromised in diet-induced obesity (DIO). The endocannabinoid system in the gut is dysregulated in DIO and participates in gut barrier function; however, whether its activity is protective or detrimental for gut barrier integrity is unclear. We used mice conditionally deficient in cannabinoid receptor subtype-1 (CB₁R) in the intestinal epithelium (intCB1-/-) to test the hypothesis that CB₁Rs in intestinal epithelial cells provide protection from diet-induced gut barrier dysfunction. Control and intCB1-/- mice were placed for eight weeks on a high-fat/sucrose Western-style diet (WD) or a low-fat/no-sucrose diet. Endocannabinoid levels and activity of their metabolic enzymes were measured in the lar2022-01-01T00:00:00Z2022 Sep2026-04-08T12:00:37.897Z2025-02-19T04:38:50.674ZS-EPMC95043033614246110.3390/ijms231810549