biostudies-literatureQi NChina Agriculture Research SystemShenyang Science and Technology Bureau Seed Industry Innovation ProjectNational Natural Science Foundation of ChinaNational Parasitic Resources Centre10771https://www.ebi.ac.uk/biostudies/studies/S-EPMC9504373biostudies-literatureUnknown23(18)Ubiquitination is a kind of post-translational modification of proteins that plays an important role in plant response to biotic and abiotic stress. The response of soybean <i>GmPUB</i> genes to soybean cyst nematode (SCN, <i>Heterodera glycines</i>) infection is largely unknown. In this study, quantitative real-time PCR (qRT-PCR) was performed to detect the relative expression of 49 <i>GmPUB</i> genes in susceptible cultivar William 82 and resistant cultivar Huipizhi after SCN inoculation. The results show that <i>GmPUB</i> genes responded to cyst nematode infection at 1 day post-inoculation (dpi), 5 dpi, 10 dpi and 15 dpi. The expression levels of <i>GmPUB16A</i>, <i>GmPUB20A</i>, <i>GmCHIPA</i>, <i>GmPUB33A</i>, <i>GmPUB23A</i> and <i>GmPUB24A</i> were dramatically changed during SCN inInternational journal of molecular sciencesFunctional Characterization of Ubiquitination Genes in the Interaction of Soybean-<i>Heterodera glycines</i>.PMC9504373CARS-04-PS133133006321-110-3-1131171569NPRC-2019-194-3031571985Qi NLiu XYan JFan HChen LZhu XXuan YWang YLei PDuan YfalseFunctional Characterization of Ubiquitination Genes in the Interaction of Soybean-<i>Heterodera glycines</i>.Ubiquitination is a kind of post-translational modification of proteins that plays an important role in plant response to biotic and abiotic stress. The response of soybean <i>GmPUB</i> genes to soybean cyst nematode (SCN, <i>Heterodera glycines</i>) infection is largely unknown. In this study, quantitative real-time PCR (qRT-PCR) was performed to detect the relative expression of 49 <i>GmPUB</i> genes in susceptible cultivar William 82 and resistant cultivar Huipizhi after SCN inoculation. The results show that <i>GmPUB</i> genes responded to cyst nematode infection at 1 day post-inoculation (dpi), 5 dpi, 10 dpi and 15 dpi. The expression levels of <i>GmPUB16A</i>, <i>GmPUB20A</i>, <i>GmCHIPA</i>, <i>GmPUB33A</i>, <i>GmPUB23A</i> and <i>GmPUB24A</i> were dramatically changed during SCN in2022-01-01T00:00:00Z2022 Sep2026-04-08T11:40:32.485Z2024-11-19T22:25:22.078ZS-EPMC95043733614267810.3390/ijms231810771