biostudies-literatureXu YHigh Talent Supporting Program of Yangzhou UniversityResearch Innovation Project of Jiangsu Province Graduate StudentsNational Natural Science Foundation of ChinaPriority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)Natural Science Foundation for Excellent Young Scholars of Jiangsu Province2056https://www.ebi.ac.uk/biostudies/studies/S-EPMC9505768biostudies-literatureUnknown14(9)Porcine reproductive and respiratory syndrome virus (PRRSV) has a strict cell tropism. In addition to the primary alveolar macrophages, PRRSV is strictly cytotropic to African green monkey kidney cells, such as MARC-145 cells; however, MARC-145 cells are not infected by most NADC30-like and NADC34-like PRRSV strains. The essential scavenger receptor CD163 has been proved to mediate productive infection of PRRSV in various non-permissive cell lines. In this study, we systematically tested the porcine CD163 stably expressing 3D4/21 cells for infections with various PRRSV strains. The results showed that the porcine CD163-expressing macrophages support the infections of PRRSV2 of lineages 1, 5, and 8, as evidenced by Western blotting, immunofluorescence assay, quantitative PCR, and virus titrVirusesCD163-Expressing Porcine Macrophages Support NADC30-like and NADC34-like PRRSV Infections.PMC95057683187245031802172BK202111603Ye MChen NZhu JLuo JSun SMeurens FWang YZheng WXu YCao QfalseCD163-Expressing Porcine Macrophages Support NADC30-like and NADC34-like PRRSV Infections.Porcine reproductive and respiratory syndrome virus (PRRSV) has a strict cell tropism. In addition to the primary alveolar macrophages, PRRSV is strictly cytotropic to African green monkey kidney cells, such as MARC-145 cells; however, MARC-145 cells are not infected by most NADC30-like and NADC34-like PRRSV strains. The essential scavenger receptor CD163 has been proved to mediate productive infection of PRRSV in various non-permissive cell lines. In this study, we systematically tested the porcine CD163 stably expressing 3D4/21 cells for infections with various PRRSV strains. The results showed that the porcine CD163-expressing macrophages support the infections of PRRSV2 of lineages 1, 5, and 8, as evidenced by Western blotting, immunofluorescence assay, quantitative PCR, and virus titr2022-01-01T00:00:00Z2022 Sep2026-04-08T11:53:11.608Z2025-02-19T00:44:15.921ZS-EPMC95057683614686210.3390/v14092056