{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["20(1)"],"submitter":["Yung Y"],"pubmed_abstract":["<h4>Objective</h4>Nowadays, different modes and timing of GnRH-agonist combined with hCG trigger, for final follicular maturation, have been described. While LH + FSH are the naturally occurring final follicular maturation trigger, hCG is commonly use during stimulated cycle, and recently the introduction of the Dual/Double trigger combines LH + FSH + hCG. In the present study we aim to investigate the messenger RNA (mRNA) expression of reproduction-related genes in human granulosa cells (GCs) exposed to the aforementioned different types and combinations of gonadotropins.<h4>Material and methods</h4>Mural GCs were obtained from follicular fluid aspirated during IVF protocol. GCs were seeded in culture for 4 days with daily medium exchange followed by administration of either hCG (1 U/ml);"],"journal":["Reproductive biology and endocrinology : RB&E"],"pagination":["144"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9508785"],"repository":["biostudies-literature"],"pubmed_title":["The in-vitro effect of gonadotropins' type and combination on Granulosa cells gene expressions."],"pmcid":["PMC9508785"],"pubmed_authors":["Haham LM","Haas J","Maydan SA","Aizer A","Maman E","Orvieto R","Tieb S","Yung Y"],"additional_accession":[]},"is_claimable":false,"name":"The in-vitro effect of gonadotropins' type and combination on Granulosa cells gene expressions.","description":"<h4>Objective</h4>Nowadays, different modes and timing of GnRH-agonist combined with hCG trigger, for final follicular maturation, have been described. While LH + FSH are the naturally occurring final follicular maturation trigger, hCG is commonly use during stimulated cycle, and recently the introduction of the Dual/Double trigger combines LH + FSH + hCG. In the present study we aim to investigate the messenger RNA (mRNA) expression of reproduction-related genes in human granulosa cells (GCs) exposed to the aforementioned different types and combinations of gonadotropins.<h4>Material and methods</h4>Mural GCs were obtained from follicular fluid aspirated during IVF protocol. GCs were seeded in culture for 4 days with daily medium exchange followed by administration of either hCG (1 U/ml);","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-05T13:31:12.081Z","creation":"2025-04-05T13:31:12.081Z"},"accession":"S-EPMC9508785","cross_references":{"pubmed":["36153536"],"doi":["10.1186/s12958-022-01017-x"]}}