biostudies-literatureUnknown20(1)Yung Y<h4>Objective</h4>Nowadays, different modes and timing of GnRH-agonist combined with hCG trigger, for final follicular maturation, have been described. While LH + FSH are the naturally occurring final follicular maturation trigger, hCG is commonly use during stimulated cycle, and recently the introduction of the Dual/Double trigger combines LH + FSH + hCG. In the present study we aim to investigate the messenger RNA (mRNA) expression of reproduction-related genes in human granulosa cells (GCs) exposed to the aforementioned different types and combinations of gonadotropins.<h4>Material and methods</h4>Mural GCs were obtained from follicular fluid aspirated during IVF protocol. GCs were seeded in culture for 4 days with daily medium exchange followed by administration of either hCG (1 U/ml);Reproductive biology and endocrinology : RB&E144https://www.ebi.ac.uk/biostudies/studies/S-EPMC9508785biostudies-literatureThe in-vitro effect of gonadotropins' type and combination on Granulosa cells gene expressions.PMC9508785Haham LMHaas JMaydan SAAizer AMaman EOrvieto RTieb SYung YfalseThe in-vitro effect of gonadotropins' type and combination on Granulosa cells gene expressions.<h4>Objective</h4>Nowadays, different modes and timing of GnRH-agonist combined with hCG trigger, for final follicular maturation, have been described. While LH + FSH are the naturally occurring final follicular maturation trigger, hCG is commonly use during stimulated cycle, and recently the introduction of the Dual/Double trigger combines LH + FSH + hCG. In the present study we aim to investigate the messenger RNA (mRNA) expression of reproduction-related genes in human granulosa cells (GCs) exposed to the aforementioned different types and combinations of gonadotropins.<h4>Material and methods</h4>Mural GCs were obtained from follicular fluid aspirated during IVF protocol. GCs were seeded in culture for 4 days with daily medium exchange followed by administration of either hCG (1 U/ml);2022-01-01T00:00:00Z2022 Sep2025-04-05T13:31:12.081Z2025-04-05T13:31:12.081ZS-EPMC95087853615353610.1186/s12958-022-01017-x