{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Antika TR"],"funding":["Ministry of Science and Technology, Taiwan"],"pagination":["10015-10025"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9508852"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["50(17)"],"pubmed_abstract":["tRNAHis guanylyltransferase (Thg1) catalyzes the 3'-5' incorporation of guanosine into position -1 (G-1) of tRNAHis. G-1 is unique to tRNAHis and is crucial for recognition by histidyl-tRNA synthetase (HisRS). Yeast Thg1 requires ATP for G-1 addition to tRNAHis opposite A73, whereas archaeal Thg1 requires either ATP or GTP for G-1 addition to tRNAHis opposite C73. Paradoxically, human Thg1 (HsThg1) can add G-1 to tRNAsHis with A73 (cytoplasmic) and C73 (mitochondrial). As N73 is immediately followed by a CCA end (positions 74-76), how HsThg1 prevents successive 3'-5' incorporation of G-1/G-2/G-3 into mitochondrial tRNAHis (tRNAmHis) through a template-dependent mechanism remains a puzzle. We showed herein that mature native human tRNAmHis indeed contains only G-1. ATP was absolutely requir"],"journal":["Nucleic acids research"],"pubmed_title":["Human Thg1 displays tRNA-inducible GTPase activity."],"pmcid":["PMC9508852"],"funding_grant_id":["110-2311-B-008-001"],"pubmed_authors":["Yeh FL","Wang TL","Chang TH","Antika TR","Nazilah KR","Lo YT","Yeh CS","Wang CC","Lee YH"],"additional_accession":[]},"is_claimable":false,"name":"Human Thg1 displays tRNA-inducible GTPase activity.","description":"tRNAHis guanylyltransferase (Thg1) catalyzes the 3'-5' incorporation of guanosine into position -1 (G-1) of tRNAHis. G-1 is unique to tRNAHis and is crucial for recognition by histidyl-tRNA synthetase (HisRS). Yeast Thg1 requires ATP for G-1 addition to tRNAHis opposite A73, whereas archaeal Thg1 requires either ATP or GTP for G-1 addition to tRNAHis opposite C73. Paradoxically, human Thg1 (HsThg1) can add G-1 to tRNAsHis with A73 (cytoplasmic) and C73 (mitochondrial). As N73 is immediately followed by a CCA end (positions 74-76), how HsThg1 prevents successive 3'-5' incorporation of G-1/G-2/G-3 into mitochondrial tRNAHis (tRNAmHis) through a template-dependent mechanism remains a puzzle. We showed herein that mature native human tRNAmHis indeed contains only G-1. ATP was absolutely requir","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2026-05-28T01:08:32.123Z","creation":"2025-04-19T22:47:07.055Z"},"accession":"S-EPMC9508852","cross_references":{"pubmed":["36107775"],"doi":["10.1093/nar/gkac768"]}}