{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Anil AT"],"funding":["IISER Mohali","DBT India Alliance Fellowship","DBT/Wellcome Trust India Alliance","Wellcome Trust","Max Planck Society, Germany"],"pagination":["10000-10014"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9508853"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["50(17)"],"pubmed_abstract":["Intron diversity facilitates regulated gene expression and alternative splicing. Spliceosomes excise introns after recognizing their splicing signals: the 5'-splice site (5'ss), branchpoint (BP) and 3'-splice site (3'ss). The latter two signals are recognized by U2 small nuclear ribonucleoprotein (snRNP) and its accessory factors (U2AFs), but longer spacings between them result in weaker splicing. Here, we show that excision of introns with a BP-distant 3'ss (e.g. rap1 intron 2) requires the ubiquitin-fold-activated splicing regulator Sde2 in Schizosaccharomyces pombe. By monitoring splicing-specific ura4 reporters in a collection of S. pombe mutants, Cay1 and Tls1 were identified as additional regulators of this process. The role of Sde2, Cay1 and Tls1 was further confirmed by increasing "],"journal":["Nucleic acids research"],"pubmed_title":["Splicing of branchpoint-distant exons is promoted by Cactin, Tls1 and the ubiquitin-fold-activated Sde2."],"pmcid":["PMC9508853"],"funding_grant_id":["IA/I/18/2/504020"],"pubmed_authors":["Anil AT","Mishra SK","Thakran P","Singh A","Pandian R","Choudhary K","Gupta P","Sharma M"],"additional_accession":[]},"is_claimable":false,"name":"Splicing of branchpoint-distant exons is promoted by Cactin, Tls1 and the ubiquitin-fold-activated Sde2.","description":"Intron diversity facilitates regulated gene expression and alternative splicing. Spliceosomes excise introns after recognizing their splicing signals: the 5'-splice site (5'ss), branchpoint (BP) and 3'-splice site (3'ss). The latter two signals are recognized by U2 small nuclear ribonucleoprotein (snRNP) and its accessory factors (U2AFs), but longer spacings between them result in weaker splicing. Here, we show that excision of introns with a BP-distant 3'ss (e.g. rap1 intron 2) requires the ubiquitin-fold-activated splicing regulator Sde2 in Schizosaccharomyces pombe. By monitoring splicing-specific ura4 reporters in a collection of S. pombe mutants, Cay1 and Tls1 were identified as additional regulators of this process. The role of Sde2, Cay1 and Tls1 was further confirmed by increasing ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2026-07-14T15:08:48.193Z","creation":"2025-04-19T22:49:37.376Z"},"accession":"S-EPMC9508853","cross_references":{"pubmed":["36095128"],"doi":["10.1093/nar/gkac769"]}}