{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hojo H"],"funding":["NIDDK NIH HHS"],"pagination":["111315"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9510047"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["40(10)"],"pubmed_abstract":["The transcriptional regulator Runx2 (runt-related transcription factor 2) has essential but distinct roles in osteoblasts and chondrocytes in skeletal development. However, Runx2-mediated regulatory mechanisms underlying the distinctive programming of osteoblasts and chondrocytes are not well understood. Here, we perform an integrative analysis to investigate Runx2-DNA binding and chromatin accessibility ex vivo using neonatal osteoblasts and chondrocytes. We find that Runx2 engages with cell-type-distinct chromatin-accessible regions, potentially interacting with different combinations of transcriptional regulators, forming cell-type-specific hotspots, and potentiating chromatin accessibility. Genetic analysis and direct cellular reprogramming studies suggest that Runx2 is essential for e"],"journal":["Cell reports"],"pubmed_title":["Runx2 regulates chromatin accessibility to direct the osteoblast program at neonatal stages."],"pmcid":["PMC9510047"],"funding_grant_id":["P01 DK056246"],"pubmed_authors":["Okada H","Saito T","Koebis M","Aiba A","Azuma T","Nakao K","Guo Q","Onodera S","Chung UI","Tanaka S","He X","Suzuki Y","McMahon AP","Ohba S","Hojo H","Seki M"],"additional_accession":[]},"is_claimable":false,"name":"Runx2 regulates chromatin accessibility to direct the osteoblast program at neonatal stages.","description":"The transcriptional regulator Runx2 (runt-related transcription factor 2) has essential but distinct roles in osteoblasts and chondrocytes in skeletal development. However, Runx2-mediated regulatory mechanisms underlying the distinctive programming of osteoblasts and chondrocytes are not well understood. Here, we perform an integrative analysis to investigate Runx2-DNA binding and chromatin accessibility ex vivo using neonatal osteoblasts and chondrocytes. We find that Runx2 engages with cell-type-distinct chromatin-accessible regions, potentially interacting with different combinations of transcriptional regulators, forming cell-type-specific hotspots, and potentiating chromatin accessibility. Genetic analysis and direct cellular reprogramming studies suggest that Runx2 is essential for e","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2026-06-01T02:26:39.808Z","creation":"2025-04-05T18:20:33.666Z"},"accession":"S-EPMC9510047","cross_references":{"pubmed":["36070691"],"doi":["10.1016/j.celrep.2022.111315"]}}