biostudies-literatureZhang KNational Key Research and Development Program of Chinae14043https://www.ebi.ac.uk/biostudies/studies/S-EPMC9511998biostudies-literatureUnknown10<h4>Background</h4>The full-length 16S rRNA sequencing can better improve the taxonomic and phylogenetic resolution compared to the partial 16S rRNA gene sequencing. The 16S-FAS-NGS (16S rRNA full-length amplicon sequencing based on a next-generation sequencing platform) technology can generate high-quality, full-length 16S rRNA gene sequences using short-read sequencers, together with assembly procedures. However there is a lack of a data analysis suite that can help process and analyze the synthetic long read data.<h4>Results</h4>Herein, we developed software named 16S-FASAS (16S full-length amplicon sequencing data analysis software) for 16S-FAS-NGS data analysis, which provided high-fidelity species-level microbiome data. 16S-FASAS consists of data quality control, <i>de novo</i> assemPeerJ16S-FASAS: an integrated pipeline for synthetic full-length 16S rRNA gene sequencing data analysis.PMC95119982020YFC2005406Lin RZhang KZhou QZhang ZChang Yfalse16S-FASAS: an integrated pipeline for synthetic full-length 16S rRNA gene sequencing data analysis.<h4>Background</h4>The full-length 16S rRNA sequencing can better improve the taxonomic and phylogenetic resolution compared to the partial 16S rRNA gene sequencing. The 16S-FAS-NGS (16S rRNA full-length amplicon sequencing based on a next-generation sequencing platform) technology can generate high-quality, full-length 16S rRNA gene sequences using short-read sequencers, together with assembly procedures. However there is a lack of a data analysis suite that can help process and analyze the synthetic long read data.<h4>Results</h4>Herein, we developed software named 16S-FASAS (16S full-length amplicon sequencing data analysis software) for 16S-FAS-NGS data analysis, which provided high-fidelity species-level microbiome data. 16S-FASAS consists of data quality control, <i>de novo</i> assem2022-01-01T00:00:00Z20222026-05-28T01:10:14.018Z2025-04-19T22:49:08.342ZS-EPMC95119983617250310.7717/peerj.14043