biostudies-literatureUnknown11(9)Zhang C<h4>Objectives</h4>Although adoptive cell therapy with T-cell receptor-engineered T cells (TCR-Ts) has mediated effective antitumor responses in several cancers, senescence of T cells could impair the therapeutic effect of TCR-Ts. Thus, it is essential to elucidate the characteristics of senescent TCR-Ts and how to subsequently improve their antitumor effect. Here, we focused on the influence of autophagy on TCR-Ts, since autophagy is tightly associated with the regulation of T-cell activation, proliferation and differentiation.<h4>Methods</h4>We first evaluated autophagy level of senescent TCR-Ts, and then the senescent TCR-Ts were expanded <i>in vitro</i> for 7 days with and without spermidine treatment, respectively. Furthermore, the proliferative potential, phenotypical characteristicsClinical & translational immunologye1419https://www.ebi.ac.uk/biostudies/studies/S-EPMC9512689biostudies-literatureAutophagic flux restoration of senescent T cells improves antitumor activity of TCR-engineered T cells.PMC9512689Shen LLi SZhou PXiao YLu ZTeng XZhang CSun YfalseAutophagic flux restoration of senescent T cells improves antitumor activity of TCR-engineered T cells.<h4>Objectives</h4>Although adoptive cell therapy with T-cell receptor-engineered T cells (TCR-Ts) has mediated effective antitumor responses in several cancers, senescence of T cells could impair the therapeutic effect of TCR-Ts. Thus, it is essential to elucidate the characteristics of senescent TCR-Ts and how to subsequently improve their antitumor effect. Here, we focused on the influence of autophagy on TCR-Ts, since autophagy is tightly associated with the regulation of T-cell activation, proliferation and differentiation.<h4>Methods</h4>We first evaluated autophagy level of senescent TCR-Ts, and then the senescent TCR-Ts were expanded <i>in vitro</i> for 7 days with and without spermidine treatment, respectively. Furthermore, the proliferative potential, phenotypical characteristics2022-01-01T00:00:00Z20222025-04-22T03:38:28.804Z2025-04-05T20:46:31.698ZS-EPMC95126893618812110.1002/cti2.1419