biostudies-literatureLou Bthe High-level Hospital Construction Project of Guangdong ProvidenceNatural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of ChinaScience and Technology Program of Guangzhou City528https://www.ebi.ac.uk/biostudies/studies/S-EPMC9512889biostudies-literatureUnknown79(10)The ciliary body critically contributes to the ocular physiology with multiple responsibilities in the production of aqueous humor, vision accommodation and intraocular immunity. Comparatively little work, however, has revealed the single-cell molecular taxonomy of the human ciliary body required for studying these functionalities. In this study, we report a comprehensive atlas of the cellular and molecular components of human ciliary body as well as their interactions using single-cell RNA sequencing (scRNAseq). Cluster analysis of the transcriptome of 14,563 individual ciliary cells from the eyes of 3 human donors identified 14 distinct cell types, including the ciliary epithelium, smooth muscle, vascular endothelial cell, immune cell and other stromal cell populations. Cell-type discrimCellular and molecular life sciences : CMLSA single-cell transcriptomic atlas of the human ciliary body.PMC95128892022A151501221030301040282070500202102010183Qian XZhang FLiu ZLiu KGu XZeng LLou BLin XGao XChen XLi JJfalseA single-cell transcriptomic atlas of the human ciliary body.The ciliary body critically contributes to the ocular physiology with multiple responsibilities in the production of aqueous humor, vision accommodation and intraocular immunity. Comparatively little work, however, has revealed the single-cell molecular taxonomy of the human ciliary body required for studying these functionalities. In this study, we report a comprehensive atlas of the cellular and molecular components of human ciliary body as well as their interactions using single-cell RNA sequencing (scRNAseq). Cluster analysis of the transcriptome of 14,563 individual ciliary cells from the eyes of 3 human donors identified 14 distinct cell types, including the ciliary epithelium, smooth muscle, vascular endothelial cell, immune cell and other stromal cell populations. Cell-type discrim2022-01-01T00:00:00Z2022 Sep2025-04-18T12:17:43.241Z2025-04-06T21:48:43.493ZS-EPMC95128893616331110.1007/s00018-022-04559-w