{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang D"],"funding":["Natural Science Foundation of Beijing Municipality","Peking University Medicine Fund of Fostering Young Scholars' Scientific & Technological Innovation A","Peking University Medicine Fund of Fostering Young Scholars’ Scientific & Technological Innovation A"],"pagination":["1697-1707"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9515061"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35(6)"],"pubmed_abstract":["Stem cells from human exfoliated deciduous teeth (SHED) are attractive seed cells for dental tissue engineering. We identified the effect of the long noncoding RNA insulin-like growth factor-binding protein 7 antisense RNA 1 (lncRNA IGFBP7-AS1) in vivo and its underlying mechanism during SHED odontogenic differentiation. IGFBP7-AS1 and insulin-like growth factor-binding protein 7 (IGFBP7) were overexpressed using lentiviruses. IGFBP7 expression was knocked down with small interfering RNA. The effect of IGFBP7-AS1 in vivo was confirmed by animal experiments. The effect of IGFBP7 on SHED odontogenic differentiation was assessed with alkaline phosphatase staining, alizarin red S staining, quantitative reverse transcription-PCR, and western blotting. The relationship between IGFBP7-AS1 and IGF"],"journal":["Human cell"],"pubmed_title":["Long non-coding RNA IGFBP7-AS1 accelerates the odontogenic differentiation of stem cells from human exfoliated deciduous teeth by regulating IGFBP7 expression."],"pmcid":["PMC9515061"],"funding_grant_id":["7222222","BMU2022PY021"],"pubmed_authors":["Zhu N","Xie F","Qin M","Wang D","Wang Y"],"additional_accession":[]},"is_claimable":false,"name":"Long non-coding RNA IGFBP7-AS1 accelerates the odontogenic differentiation of stem cells from human exfoliated deciduous teeth by regulating IGFBP7 expression.","description":"Stem cells from human exfoliated deciduous teeth (SHED) are attractive seed cells for dental tissue engineering. We identified the effect of the long noncoding RNA insulin-like growth factor-binding protein 7 antisense RNA 1 (lncRNA IGFBP7-AS1) in vivo and its underlying mechanism during SHED odontogenic differentiation. IGFBP7-AS1 and insulin-like growth factor-binding protein 7 (IGFBP7) were overexpressed using lentiviruses. IGFBP7 expression was knocked down with small interfering RNA. The effect of IGFBP7-AS1 in vivo was confirmed by animal experiments. The effect of IGFBP7 on SHED odontogenic differentiation was assessed with alkaline phosphatase staining, alizarin red S staining, quantitative reverse transcription-PCR, and western blotting. The relationship between IGFBP7-AS1 and IGF","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-05-18T13:41:09.321Z","creation":"2024-10-18T22:47:27.826Z"},"accession":"S-EPMC9515061","cross_references":{"pubmed":["36038801"],"doi":["10.1007/s13577-022-00763-9"]}}