biostudies-literatureUnknown12(15)Wang Y<b>Rationale:</b> Hyperactivation of Hippo-Yes-associated protein (YAP) signaling pathway governs tumorigenesis of gastric cancer (GC). Here we reveal that minichromosome maintenance complex component 6 (MCM6) is a critical transcriptional target of YAP in GC. We aim to investigate the function, mechanism of action, and clinical implication of MCM6 in GC. <b>Methods:</b> The downstream targets of YAP were screened by RNA sequencing (RNA-seq) and microarray, and further validated by chromatin immunoprecipitation PCR and luciferase reporter assays. The clinical implication of MCM6 was assessed in multiple GC cohorts. Biological function of MCM6 was evaluated <i>in vitro</i>, in patient-derived organoids, and <i>in vivo</i>. RNA-seq was performed to unravel downstream signaling of MCM6. PotenTheranostics6509-6526https://www.ebi.ac.uk/biostudies/studies/S-EPMC9516235biostudies-literatureMCM6 is a critical transcriptional target of YAP to promote gastric tumorigenesis and serves as a therapeutic target.PMC9516235Wu WKKKang WZhang JMa BBYLo KWWong CCChen HYu JChen BLiu WZhou ZTo KFZhang YCheung AHKLiang LChan MWYCheng ASLWang YYan HfalseMCM6 is a critical transcriptional target of YAP to promote gastric tumorigenesis and serves as a therapeutic target.<b>Rationale:</b> Hyperactivation of Hippo-Yes-associated protein (YAP) signaling pathway governs tumorigenesis of gastric cancer (GC). Here we reveal that minichromosome maintenance complex component 6 (MCM6) is a critical transcriptional target of YAP in GC. We aim to investigate the function, mechanism of action, and clinical implication of MCM6 in GC. <b>Methods:</b> The downstream targets of YAP were screened by RNA sequencing (RNA-seq) and microarray, and further validated by chromatin immunoprecipitation PCR and luciferase reporter assays. The clinical implication of MCM6 was assessed in multiple GC cohorts. Biological function of MCM6 was evaluated <i>in vitro</i>, in patient-derived organoids, and <i>in vivo</i>. RNA-seq was performed to unravel downstream signaling of MCM6. Poten2022-01-01T00:00:00Z20222026-05-31T15:56:46.352Z2025-04-04T12:45:29.307ZS-EPMC95162353618559810.7150/thno.75431