biostudies-literatureGoonawardane NWellcome Truste0081822https://www.ebi.ac.uk/biostudies/studies/S-EPMC9517736biostudies-literatureUnknown96(18)Tick-borne encephalitis virus (TBEV) is an important human arthropod-borne virus that causes tick-borne encephalitis (TBE) in humans. TBEV acutely infects the central nervous system (CNS), leading to neurological symptoms of various severity. No therapeutics are currently available for TBEV-associated disease. Virus strains of various pathogenicity have been described, although the basis of their diverse clinical outcome remains undefined. Work with infectious TBEV requires high-level biocontainment, meaning model systems that can recapitulate the virus life cycle are highly sought. Here, we report the generation of a self-replicating, noninfectious TBEV replicon used to study properties of high (Hypr) and low (Vs) pathogenic TBEV isolates. Using a Spinach2 RNA aptamer and luciferase reporJournal of virologyIdentification of Host Factors Differentially Induced by Clinically Diverse Strains of Tick-Borne Encephalitis Virus.PMC9517736096670/Z/11/Z104918/Z/14/ZHarris MGoonawardane NUpstone LJones IMfalseIdentification of Host Factors Differentially Induced by Clinically Diverse Strains of Tick-Borne Encephalitis Virus.Tick-borne encephalitis virus (TBEV) is an important human arthropod-borne virus that causes tick-borne encephalitis (TBE) in humans. TBEV acutely infects the central nervous system (CNS), leading to neurological symptoms of various severity. No therapeutics are currently available for TBEV-associated disease. Virus strains of various pathogenicity have been described, although the basis of their diverse clinical outcome remains undefined. Work with infectious TBEV requires high-level biocontainment, meaning model systems that can recapitulate the virus life cycle are highly sought. Here, we report the generation of a self-replicating, noninfectious TBEV replicon used to study properties of high (Hypr) and low (Vs) pathogenic TBEV isolates. Using a Spinach2 RNA aptamer and luciferase repor2022-01-01T00:00:00Z2022 Sep2025-04-04T19:40:41.312Z2024-11-07T11:59:36.173ZS-EPMC95177363609851310.1128/jvi.00818-22