biostudies-literatureUnknown19(17)Alshammari AThe misuse of antibiotics in our daily lives has led to the emergence of antimicrobial resistance. As a result, many antibiotics are becoming ineffective. This phenomenon is linked with high rates of mortality and morbidity. Therefore, new approaches are required to address this major health issue. <i>Leptotrichia buccalis</i> is a Gram-negative, rod-shaped bacterium which normally resides in the oral and vaginal cavities. It is an emerging bacterial pathogen which is developing new antibiotic-resistance mechanisms. No approved vaccine is available against this pathogen, which is a cause for growing concern. In this study, an in silico-based, multi-epitopes vaccine against this pathogen was designed by applying reverse vaccinology and immunoinformatic approaches. Of a total of 2193 predictInternational journal of environmental research and public health10742https://www.ebi.ac.uk/biostudies/studies/S-EPMC9518150biostudies-literatureNovel Chimeric Vaccine Candidate Development against <i>Leptotrichia buccalis</i>.PMC9518150Muhseen ZTUllah AArshad MAlharbi MAshfaq UAUd-Din MAhmad SAli NAlasmari AFAlshammari AfalseNovel Chimeric Vaccine Candidate Development against <i>Leptotrichia buccalis</i>.The misuse of antibiotics in our daily lives has led to the emergence of antimicrobial resistance. As a result, many antibiotics are becoming ineffective. This phenomenon is linked with high rates of mortality and morbidity. Therefore, new approaches are required to address this major health issue. <i>Leptotrichia buccalis</i> is a Gram-negative, rod-shaped bacterium which normally resides in the oral and vaginal cavities. It is an emerging bacterial pathogen which is developing new antibiotic-resistance mechanisms. No approved vaccine is available against this pathogen, which is a cause for growing concern. In this study, an in silico-based, multi-epitopes vaccine against this pathogen was designed by applying reverse vaccinology and immunoinformatic approaches. Of a total of 2193 predict2022-01-01T00:00:00Z2022 Aug2026-04-08T11:55:03.153Z2025-02-19T02:24:33.708ZS-EPMC95181503607846210.3390/ijerph191710742