{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cui S"],"funding":["AO Foundation","AOSpine"],"pagination":["e1215"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9520770"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(3)"],"pubmed_abstract":["<h4>Background</h4>During the intervertebral disc (IVD) degeneration process, initial degenerative events occur at the extracellular matrix level, with the appearance of neoepitope peptides formed by the cleavage of aggrecan and collagen. This study aims to elucidate the spatial and temporal alterations of aggrecan and collagen neoepitope level during IVD degeneration.<h4>Methods</h4>Bovine caudal IVDs were cultured under four different conditions to mimic different degenerative situations. Samples cultured after 1- or 8-days were collected for analysis. Human IVD samples were obtained from patients diagnosed with lumbar disc herniation (LDH) or adolescent idiopathic scoliosis (AIS). After immunohistochemical (IHC) staining of Aggrecanase Cleaved C-terminus Aggrecan Neoepitope (NB100), MMP"],"journal":["JOR spine"],"pubmed_title":["Neoepitope fragments as biomarkers for different phenotypes of intervertebral disc degeneration."],"pmcid":["PMC9520770"],"funding_grant_id":["NeoDisc","Theranostic"],"pubmed_authors":["Li W","Neidlinger-Wilke C","Li Z","Ouyang H","Richards RG","Haglund L","Cui S","Wilke HJ","Grad S","Alini M","Teixeira GQ"],"additional_accession":[]},"is_claimable":false,"name":"Neoepitope fragments as biomarkers for different phenotypes of intervertebral disc degeneration.","description":"<h4>Background</h4>During the intervertebral disc (IVD) degeneration process, initial degenerative events occur at the extracellular matrix level, with the appearance of neoepitope peptides formed by the cleavage of aggrecan and collagen. This study aims to elucidate the spatial and temporal alterations of aggrecan and collagen neoepitope level during IVD degeneration.<h4>Methods</h4>Bovine caudal IVDs were cultured under four different conditions to mimic different degenerative situations. Samples cultured after 1- or 8-days were collected for analysis. Human IVD samples were obtained from patients diagnosed with lumbar disc herniation (LDH) or adolescent idiopathic scoliosis (AIS). After immunohistochemical (IHC) staining of Aggrecanase Cleaved C-terminus Aggrecan Neoepitope (NB100), MMP","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-19T19:43:02.838Z","creation":"2025-04-19T19:43:02.838Z"},"accession":"S-EPMC9520770","cross_references":{"pubmed":["36203866"],"doi":["10.1002/jsp2.1215"]}}