biostudies-literatureChen TNatural Science Foundation of Tianjin CityNational Natural Science Foundation of China335https://www.ebi.ac.uk/biostudies/studies/S-EPMC9520944biostudies-literatureUnknown20(1)<h4>Background</h4>Long noncoding RNAs (lncRNAs) have emerged as important regulators in a variety of human diseases. The dysregulation of liver sinusoidal endothelial cell (LSEC) phenotype is a critical early event in the fibrotic process. However, the biological function of lncRNAs in LSEC still remains unclear.<h4>Methods</h4>The expression level of lncRNA Airn was evaluated in both human fibrotic livers and serums, as well as mouse fibrotic livers. Gain- and loss-of-function experiments were performed to detect the effect of Airn on LSEC differentiation and hepatic stellate cell (HSC) activation in liver fibrosis. Furthermore, RIP, RNA pull-down-immunoblotting, and ChIP experiments were performed to explore the underlying mechanisms of Airn.<h4>Results</h4>We have identified Airn was sBMC medicineLncRNA Airn maintains LSEC differentiation to alleviate liver fibrosis via the KLF2-eNOS-sGC pathway.PMC9520944819713318187042982170630321711258180054219JCZDJC36700Zhang KHu ZHan XDu XYao QShi ZHan TZhao YMeng XZhao SZheng LLin HHong WChen TfalseLncRNA Airn maintains LSEC differentiation to alleviate liver fibrosis via the KLF2-eNOS-sGC pathway.<h4>Background</h4>Long noncoding RNAs (lncRNAs) have emerged as important regulators in a variety of human diseases. The dysregulation of liver sinusoidal endothelial cell (LSEC) phenotype is a critical early event in the fibrotic process. However, the biological function of lncRNAs in LSEC still remains unclear.<h4>Methods</h4>The expression level of lncRNA Airn was evaluated in both human fibrotic livers and serums, as well as mouse fibrotic livers. Gain- and loss-of-function experiments were performed to detect the effect of Airn on LSEC differentiation and hepatic stellate cell (HSC) activation in liver fibrosis. Furthermore, RIP, RNA pull-down-immunoblotting, and ChIP experiments were performed to explore the underlying mechanisms of Airn.<h4>Results</h4>We have identified Airn was s2022-01-01T00:00:00Z2022 Sep2026-05-27T19:01:01.087Z2025-02-19T01:55:45.916ZS-EPMC95209443617160610.1186/s12916-022-02523-w