biostudies-literatureUnknown13Zhang DNational Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaProgram for Changjiang Scholars and Innovative Research Team in UniversityThe study of preimplantation development is of great significance to reproductive biology and regenerative medicine. With the development of high-throughput deep sequencing technology, it has been found that lncRNAs play a very important role in the regulation of embryonic development. In this study, key lncRNAs that regulate embryonic development were screened by analyzing the expression pattern of lncRNAs in porcine <i>in vivo</i> fertilization (IVV) embryos. By knocking down lncRNA expression in <i>in vitro</i> fertilization (IVF) embryos, we investigated its function and mechanism of regulating embryonic development. The results showed that the expression pattern of lncRNA was consistent with the time of gene activation. The lncRNAs were highly expressed in the 4-cell to blastocyst staFrontiers in physiology971965https://www.ebi.ac.uk/biostudies/studies/S-EPMC9523245biostudies-literatureLncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene <i>SIN3A</i>.PMC9523245Shi LZhang DLi ZZhai YAn XLi QWu DZhou YZhang SHuang RKong XYu HfalseLncRNA affects epigenetic reprogramming of porcine embryo development by regulating global epigenetic modification and the downstream gene <i>SIN3A</i>.The study of preimplantation development is of great significance to reproductive biology and regenerative medicine. With the development of high-throughput deep sequencing technology, it has been found that lncRNAs play a very important role in the regulation of embryonic development. In this study, key lncRNAs that regulate embryonic development were screened by analyzing the expression pattern of lncRNAs in porcine <i>in vivo</i> fertilization (IVV) embryos. By knocking down lncRNA expression in <i>in vitro</i> fertilization (IVF) embryos, we investigated its function and mechanism of regulating embryonic development. The results showed that the expression pattern of lncRNA was consistent with the time of gene activation. The lncRNAs were highly expressed in the 4-cell to blastocyst sta2022-01-01T00:00:00Z20222026-04-08T18:07:37.044Z2025-04-05T20:45:34.885ZS-EPMC95232453618779110.3389/fphys.2022.971965