{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yu F"],"funding":["Science Fund for Distinguished Young Scholars of Guangxi Province","Natural Science Foundation of Guangxi Province","National Natural Science Foundation of China","Science and Technology Planning Project of Nanning Qingxiu District"],"pagination":["115"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9524076"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(1)"],"pubmed_abstract":["Background
As the increasing mortality and incidence of lung cancer (LC), there is an urgent need to discover novel treatment agent. In this study, we aimed to investigate the anti-LC effects of nitidine chloride (NC), a small molecular compound extracted from Chinese herbal medicine, while detailing its underlying mechanisms.Methods
Cell viability was detected by MTT assays and five cell death inhibitors, including ferrostatin-1 (Fer-1), Z-VAD-FMK, necrostatin-1 (Nec-1), disulfiram (DSF) and IM-54 were used to explore the type of cell death induced by NC. The microscopic features of NC-induced pyroptosis were assessed by transmission electron microscopy (TEM) and the pyroptotic-related proteins such as caspase and gasdermin family, were examined by western blot. Network ph"],"journal":["Chinese medicine"],"pubmed_title":["Nitidine chloride induces caspase 3/GSDME-dependent pyroptosis by inhibting PI3K/Akt pathway in lung cancer."],"pmcid":["PMC9524076"],"funding_grant_id":["No.2021013","No.2022GXNSFBA035590","No. 81860649","No.82160698"],"pubmed_authors":["Yang J","Tan W","Chen Z","He J","Luo Z","Yu F","Deng Z","Shen X","Wang J","Mo X"],"additional_accession":[]},"is_claimable":false,"name":"Nitidine chloride induces caspase 3/GSDME-dependent pyroptosis by inhibting PI3K/Akt pathway in lung cancer.","description":"Background
As the increasing mortality and incidence of lung cancer (LC), there is an urgent need to discover novel treatment agent. In this study, we aimed to investigate the anti-LC effects of nitidine chloride (NC), a small molecular compound extracted from Chinese herbal medicine, while detailing its underlying mechanisms.Methods
Cell viability was detected by MTT assays and five cell death inhibitors, including ferrostatin-1 (Fer-1), Z-VAD-FMK, necrostatin-1 (Nec-1), disulfiram (DSF) and IM-54 were used to explore the type of cell death induced by NC. The microscopic features of NC-induced pyroptosis were assessed by transmission electron microscopy (TEM) and the pyroptotic-related proteins such as caspase and gasdermin family, were examined by western blot. Network ph","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-19T06:33:56.996Z","creation":"2025-02-19T00:24:13.158Z"},"accession":"S-EPMC9524076","cross_references":{"pubmed":["36175965"],"doi":["10.1186/s13020-022-00671-y"]}}