biostudies-literatureUnknown17(9)Myers FThe COVID-19 pandemic has highlighted the need for broader access to molecular diagnostics. Colorimetric isothermal nucleic acid amplification assays enable simplified instrumentation over more conventional PCR diagnostic assays and, as such, represent a promising approach for addressing this need. In particular, colorimetric LAMP (loop-mediated isothermal amplification) has received a great deal of interest recently. However, there do not currently exist robust instruments for performing these kinds of assays in high throughput with real-time readout of amplification signals. To address this need, we developed LARI, the LAMP Assay Reader Instrument. We have deployed over 50 LARIs for routine use in R&D and production environments, with over 12,000 assays run to date. In this paper, we prePloS onehttps://www.ebi.ac.uk/biostudies/studies/S-EPMC9524685biostudies-literatureA robust, low-cost instrument for real-time colorimetric isothermal nucleic acid amplificationPMC9524685El Khaja RMcGee MChatterjee TMoffatt BMarwaha GMyers FMitra DfalseA robust, low-cost instrument for real-time colorimetric isothermal nucleic acid amplificationThe COVID-19 pandemic has highlighted the need for broader access to molecular diagnostics. Colorimetric isothermal nucleic acid amplification assays enable simplified instrumentation over more conventional PCR diagnostic assays and, as such, represent a promising approach for addressing this need. In particular, colorimetric LAMP (loop-mediated isothermal amplification) has received a great deal of interest recently. However, there do not currently exist robust instruments for performing these kinds of assays in high throughput with real-time readout of amplification signals. To address this need, we developed LARI, the LAMP Assay Reader Instrument. We have deployed over 50 LARIs for routine use in R&D and production environments, with over 12,000 assays run to date. In this paper, we pre2022-01-01T00:00:00Z2022 Jan2025-04-04T12:04:34.706Z2025-02-19T00:23:47.788ZS-EPMC9524685