{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lyu Z"],"funding":["NIAID NIH HHS","Foundation for the National Institutes of Health","NIGMS NIH HHS"],"pagination":["5751"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9525312"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["FtsN plays an essential role in promoting the inward synthesis of septal peptidoglycan (sPG) by the FtsWI complex during bacterial cell division. How it achieves this role is unclear. Here we use single-molecule tracking to investigate FtsN's dynamics during sPG synthesis in E. coli. We show that septal FtsN molecules move processively at ~9 nm s<sup>-1</sup>, the same as FtsWI molecules engaged in sPG synthesis (termed sPG-track), but much slower than the ~30 nm s<sup>-1</sup> speed of inactive FtsWI molecules coupled to FtsZ's treadmilling dynamics (termed FtsZ-track). Importantly, processive movement of FtsN is exclusively coupled to sPG synthesis and is required to maintain active sPG synthesis by FtsWI. Our findings indicate that FtsN is part of the FtsWI sPG synthesis complex, and th"],"journal":["Nature communications"],"pubmed_title":["FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli."],"pmcid":["PMC9525312"],"funding_grant_id":["R01 GM086447","GM125656","R35 GM136436","R01GM125656","R01GM086447","R01 GM125656","T32 AI007511","T32AI007511","R35GM136436","GM007445"],"pubmed_authors":["Yang X","Lyu Z","Weiss DS","McQuillen R","Xiao J","McCausland JW","Kaus GM","Yahashiri A"],"additional_accession":[]},"is_claimable":false,"name":"FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli.","description":"FtsN plays an essential role in promoting the inward synthesis of septal peptidoglycan (sPG) by the FtsWI complex during bacterial cell division. How it achieves this role is unclear. Here we use single-molecule tracking to investigate FtsN's dynamics during sPG synthesis in E. coli. We show that septal FtsN molecules move processively at ~9 nm s<sup>-1</sup>, the same as FtsWI molecules engaged in sPG synthesis (termed sPG-track), but much slower than the ~30 nm s<sup>-1</sup> speed of inactive FtsWI molecules coupled to FtsZ's treadmilling dynamics (termed FtsZ-track). Importantly, processive movement of FtsN is exclusively coupled to sPG synthesis and is required to maintain active sPG synthesis by FtsWI. Our findings indicate that FtsN is part of the FtsWI sPG synthesis complex, and th","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-26T12:00:30.717Z","creation":"2025-02-19T00:24:02.385Z"},"accession":"S-EPMC9525312","cross_references":{"pubmed":["36180460"],"doi":["10.1038/s41467-022-33404-8"]}}