biostudies-literatureHan ZScience and Technology Commission of Shanghai MunicipalityNational Natural Science Foundation of China100427https://www.ebi.ac.uk/biostudies/studies/S-EPMC9526170biostudies-literatureUnknown16Wound healing and angiogenesis remain challenges for both clinical and experimental research worldwide. Periosteum-derived extracellular vesicles (P-sEVs) delivered by hydrogel dressings provide a potential strategy for wound defects to promote fast healing. In this study, we designed a NAGA/GelMA/Laponite/glycerol hydrogel wound dressing that can release P-sEVs to accelerate angiogenesis and wound healing (named P-sEVs@hydrogel) (<i>N</i>-acryloyl glycinamide, NAGA). The wound dressing showed multiple functions, including efficient angiogenesis, tissue adhesion and a physical barrier. P-sEVs significantly enhanced the proliferation, migration, and tube formation of endothelial cells <i>in vitro</i>. The results of <i>in vivo</i> experiments showed that P-sEVs@hydrogel accelerates the healing of a full-thickness defect wound model by stimulating the angiogenic process. The improved cell proliferation, tissue formation, remodeling, and re-epithelialization possibly resulted in the fast healing. This study shows that multifunctional hydrogel dressing combined with bioactive molecules can achieve fast and satisfactory wound healing in full-thickness wound defects and other related wounds.Materials today. BioEfficient angiogenesis-based wound healing through hydrogel dressing with extracellular vesicles release.PMC9526170820724222020PJD0428157217852075324Li XLi ZZhang ZDong LLi AFu LHan ZfalseEfficient angiogenesis-based wound healing through hydrogel dressing with extracellular vesicles release.Wound healing and angiogenesis remain challenges for both clinical and experimental research worldwide. Periosteum-derived extracellular vesicles (P-sEVs) delivered by hydrogel dressings provide a potential strategy for wound defects to promote fast healing. In this study, we designed a NAGA/GelMA/Laponite/glycerol hydrogel wound dressing that can release P-sEVs to accelerate angiogenesis and wound healing (named P-sEVs@hydrogel) (<i>N</i>-acryloyl glycinamide, NAGA). The wound dressing showed multiple functions, including efficient angiogenesis, tissue adhesion and a physical barrier. P-sEVs significantly enhanced the proliferation, migration, and tube formation of endothelial cells <i>in vitro</i>. The results of <i>in vivo</i> experiments showed that P-sEVs@hydrogel accelerates the healing of a full-thickness defect wound model by stimulating the angiogenic process. The improved cell proliferation, tissue formation, remodeling, and re-epithelialization possibly resulted in the fast healing. This study shows that multifunctional hydrogel dressing combined with bioactive molecules can achieve fast and satisfactory wound healing in full-thickness wound defects and other related wounds.2022-01-01T00:00:00Z2022 Dec2025-04-19T11:32:05.812Z2025-02-19T00:23:41.332ZS-EPMC95261703619334410.1016/j.mtbio.2022.100427