{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mitsiadis TA"],"funding":["Deutsche Forschungsgemeinschaft","Universität Zürich"],"pagination":["105154"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9526176"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(10)"],"pubmed_abstract":["The disintegrin and metalloproteinase Adam10 is a membrane-bound sheddase that regulates Notch signaling and ensures epidermal integrity. To address the function of Adam10 in the continuously growing incisors, we used Keratin14 <sup><i>Cre</i>/+</sup>;Adam10 <sup><i>fl/fl</i></sup> transgenic mice, in which Adam10 is conditionally deleted in the dental epithelium. Keratin14 <sup><i>Cre</i>/+</sup>;Adam10 <sup><i>fl/fl</i></sup> mice exhibited severe abnormalities, including defective enamel formation reminiscent of human enamel pathologies. Histological analyses of mutant incisors revealed absence of stratum intermedium, and severe disorganization of enamel-secreting ameloblasts. <i>In situ</i> hybridization and immunostaining analyses in the Keratin14 <sup><i>Cre</i>/+</sup>;Adam10 <sup><"],"journal":["iScience"],"pubmed_title":["Adam10-dependent Notch signaling establishes dental epithelial cell boundaries required for enamel formation."],"pmcid":["PMC9526176"],"funding_grant_id":["SFB877-A3"],"pubmed_authors":["Jimenez-Rojo L","Pagella P","Mitsiadis TA","Weber S","Saftig P","Balic A"],"additional_accession":[]},"is_claimable":false,"name":"Adam10-dependent Notch signaling establishes dental epithelial cell boundaries required for enamel formation.","description":"The disintegrin and metalloproteinase Adam10 is a membrane-bound sheddase that regulates Notch signaling and ensures epidermal integrity. To address the function of Adam10 in the continuously growing incisors, we used Keratin14 <sup><i>Cre</i>/+</sup>;Adam10 <sup><i>fl/fl</i></sup> transgenic mice, in which Adam10 is conditionally deleted in the dental epithelium. Keratin14 <sup><i>Cre</i>/+</sup>;Adam10 <sup><i>fl/fl</i></sup> mice exhibited severe abnormalities, including defective enamel formation reminiscent of human enamel pathologies. Histological analyses of mutant incisors revealed absence of stratum intermedium, and severe disorganization of enamel-secreting ameloblasts. <i>In situ</i> hybridization and immunostaining analyses in the Keratin14 <sup><i>Cre</i>/+</sup>;Adam10 <sup><","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-19T11:33:04.544Z","creation":"2025-02-19T00:23:29.937Z"},"accession":"S-EPMC9526176","cross_references":{"pubmed":["36193048"],"doi":["10.1016/j.isci.2022.105154"]}}