{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10"],"submitter":["Liu X"],"pubmed_abstract":["This work explored the mechanism of the effect of breast-cancer susceptibility gene 1 (<i>BRCA1</i>) on the metabolic characteristics of breast cancer cells, including the Warburg effect and its specific signaling. We transfected MCF-7 cells with a <i>BRCA1</i>-encoding LXSN plasmid or PKM2 siRNA and examined cancer cell metabolism using annexin V staining, inhibitory concentration determination, Western blotting, glucose uptake and lactic acid content measurements, and Transwell assays to assess glycolytic activity, cell apoptosis, and migration, and sensitivity to anti-cancer treatment. The <i>BRCA1</i>-expressing MCF-7 cells demonstrated low PKM2 expression and decreased glycolytic activity (downregulated hexokinase 2 (HK2) expression, upregulated isocitrate dehydrogenase 1 (IDH1) expre"],"journal":["PeerJ"],"pagination":["e14052"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9526413"],"repository":["biostudies-literature"],"pubmed_title":["BRCA1 overexpression attenuates breast cancer cell growth and migration by regulating the pyruvate kinase M2-mediated Warburg effect <i>via</i> the PI3K/AKT signaling pathway."],"pmcid":["PMC9526413"],"pubmed_authors":["Liu X","Liu H","Zeng L","Lv Y"],"additional_accession":[]},"is_claimable":false,"name":"BRCA1 overexpression attenuates breast cancer cell growth and migration by regulating the pyruvate kinase M2-mediated Warburg effect <i>via</i> the PI3K/AKT signaling pathway.","description":"This work explored the mechanism of the effect of breast-cancer susceptibility gene 1 (<i>BRCA1</i>) on the metabolic characteristics of breast cancer cells, including the Warburg effect and its specific signaling. We transfected MCF-7 cells with a <i>BRCA1</i>-encoding LXSN plasmid or PKM2 siRNA and examined cancer cell metabolism using annexin V staining, inhibitory concentration determination, Western blotting, glucose uptake and lactic acid content measurements, and Transwell assays to assess glycolytic activity, cell apoptosis, and migration, and sensitivity to anti-cancer treatment. The <i>BRCA1</i>-expressing MCF-7 cells demonstrated low PKM2 expression and decreased glycolytic activity (downregulated hexokinase 2 (HK2) expression, upregulated isocitrate dehydrogenase 1 (IDH1) expre","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-19T11:31:16.482Z","creation":"2025-04-19T11:31:16.482Z"},"accession":"S-EPMC9526413","cross_references":{"pubmed":["36193432"],"doi":["10.7717/peerj.14052"]}}