biostudies-literatureUnknown10Liu XThis work explored the mechanism of the effect of breast-cancer susceptibility gene 1 (<i>BRCA1</i>) on the metabolic characteristics of breast cancer cells, including the Warburg effect and its specific signaling. We transfected MCF-7 cells with a <i>BRCA1</i>-encoding LXSN plasmid or PKM2 siRNA and examined cancer cell metabolism using annexin V staining, inhibitory concentration determination, Western blotting, glucose uptake and lactic acid content measurements, and Transwell assays to assess glycolytic activity, cell apoptosis, and migration, and sensitivity to anti-cancer treatment. The <i>BRCA1</i>-expressing MCF-7 cells demonstrated low PKM2 expression and decreased glycolytic activity (downregulated hexokinase 2 (HK2) expression, upregulated isocitrate dehydrogenase 1 (IDH1) exprePeerJe14052https://www.ebi.ac.uk/biostudies/studies/S-EPMC9526413biostudies-literatureBRCA1 overexpression attenuates breast cancer cell growth and migration by regulating the pyruvate kinase M2-mediated Warburg effect <i>via</i> the PI3K/AKT signaling pathway.PMC9526413Liu XLiu HZeng LLv YfalseBRCA1 overexpression attenuates breast cancer cell growth and migration by regulating the pyruvate kinase M2-mediated Warburg effect <i>via</i> the PI3K/AKT signaling pathway.This work explored the mechanism of the effect of breast-cancer susceptibility gene 1 (<i>BRCA1</i>) on the metabolic characteristics of breast cancer cells, including the Warburg effect and its specific signaling. We transfected MCF-7 cells with a <i>BRCA1</i>-encoding LXSN plasmid or PKM2 siRNA and examined cancer cell metabolism using annexin V staining, inhibitory concentration determination, Western blotting, glucose uptake and lactic acid content measurements, and Transwell assays to assess glycolytic activity, cell apoptosis, and migration, and sensitivity to anti-cancer treatment. The <i>BRCA1</i>-expressing MCF-7 cells demonstrated low PKM2 expression and decreased glycolytic activity (downregulated hexokinase 2 (HK2) expression, upregulated isocitrate dehydrogenase 1 (IDH1) expre2022-01-01T00:00:00Z20222025-04-19T11:31:16.482Z2025-04-19T11:31:16.482ZS-EPMC95264133619343210.7717/peerj.14052