{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ling JP"],"funding":["U.S. Department of Health & Human Services | NIH | National Eye Institute","NEI NIH HHS","Research to Prevent Blindness","NHLBI NIH HHS","NIMH NIH HHS","NINDS NIH HHS","U.S. Department of Health & Human Services | NIH | National Institute of Mental Health","NIH HHS"],"pagination":["5773"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9526712"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Precise and reliable cell-specific gene delivery remains technically challenging. Here we report a splicing-based approach for controlling gene expression whereby separate translational reading frames are coupled to the inclusion or exclusion of mutated, frameshifting cell-specific alternative exons. Candidate exons are identified by analyzing thousands of publicly available RNA sequencing datasets and filtering by cell specificity, conservation, and local intron length. This method, which we denote splicing-linked expression design (SLED), can be combined in a Boolean manner with existing techniques such as minipromoters and viral capsids. SLED can use strong constitutive promoters, without sacrificing precision, by decoupling the tradeoff between promoter strength and selectivity. AAV-pa"],"journal":["Nature communications"],"pubmed_title":["Cell-specific regulation of gene expression using splicing-dependent frameshifting."],"pmcid":["PMC9526712"],"funding_grant_id":["P30 EY025585","R01 HL159306","P30 EY001765","R37 NS036715","R01 EY033103","R24 EY027283","S10 OD026859","T32 OD011089","T32 EY007143","RF1MH123237","K08 EY027093","R24EY027283","Stein Innovation Award","RF1 MH123237"],"pubmed_authors":["Bergles D","Karantanos T","Yu M","Jiang L","Bygrave AM","Teodorescu P","Bowden KD","Kanold PO","Dalton WB","Rajaii F","Dubner B","Nielsen KJ","Singh MS","Blackshaw S","Dongmo R","Liu Y","Parker P","Ling JP","Duncan LH","Smith JJ","Helmenstine E","Sun S","Leavey PJ","Huganir RL","Santiago CP","Trinh VT","Ghiaur G","Babola TA","Vistein R","Langmead B","Li Y","Taneja K","Carmen-Orozco RP","Han J","Peachey N","Gimmen MY"],"additional_accession":[]},"is_claimable":false,"name":"Cell-specific regulation of gene expression using splicing-dependent frameshifting.","description":"Precise and reliable cell-specific gene delivery remains technically challenging. Here we report a splicing-based approach for controlling gene expression whereby separate translational reading frames are coupled to the inclusion or exclusion of mutated, frameshifting cell-specific alternative exons. Candidate exons are identified by analyzing thousands of publicly available RNA sequencing datasets and filtering by cell specificity, conservation, and local intron length. This method, which we denote splicing-linked expression design (SLED), can be combined in a Boolean manner with existing techniques such as minipromoters and viral capsids. SLED can use strong constitutive promoters, without sacrificing precision, by decoupling the tradeoff between promoter strength and selectivity. AAV-pa","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-04T09:30:27.031Z","creation":"2025-02-19T00:23:38.354Z"},"accession":"S-EPMC9526712","cross_references":{"pubmed":["36182931"],"doi":["10.1038/s41467-022-33523-2"]}}