biostudies-literatureHe ZMedico- Engineering Cooperation Funds from University of Electronic Science and Technology of ChinaMedico- Engineering Cooperation Funds from University of Electronic Science and Technology of ChinaFoundation for Sichuan Provincial People’s HospitalFoundation for Sichuan Provincial People's Hospital443https://www.ebi.ac.uk/biostudies/studies/S-EPMC9526964biostudies-literatureUnknown20(1)<h4>Background</h4>Gliomas account for about 80% of all malignant brain and other central nervous system (CNS) tumors. Temozolomide (TMZ) resistance represents a major treatment hurdle. Adrenomedullin (ADM) has been reported to induce glioblastoma cell growth.<h4>Methods</h4>Cell viability was measured using the CCK-8 assay. The apoptosis analysis was performed using the Annexin V-FITC Apoptosis Detection Kit. The mitochondrial membrane potential was determined by JC-1 staining. A nude mouse tumor assay was used to detect tumor formation. Hematoxylin and eosin (H&E) and immunohistochemical (IHC) staining were performed in tissue sections. Activation of Akt and Erk and expression of apoptosis-related proteins were determined by immunoblotting.<h4>Results</h4>ADM expression has been found upJournal of translational medicinemiR-1297 sensitizes glioma cells to temozolomide (TMZ) treatment through targeting adrenomedullin (ADM).PMC95269642021LY03ZYGX2021YGLH209Tang JLiu LHe ZHu JChen LLiu PCao DCheng MfalsemiR-1297 sensitizes glioma cells to temozolomide (TMZ) treatment through targeting adrenomedullin (ADM).<h4>Background</h4>Gliomas account for about 80% of all malignant brain and other central nervous system (CNS) tumors. Temozolomide (TMZ) resistance represents a major treatment hurdle. Adrenomedullin (ADM) has been reported to induce glioblastoma cell growth.<h4>Methods</h4>Cell viability was measured using the CCK-8 assay. The apoptosis analysis was performed using the Annexin V-FITC Apoptosis Detection Kit. The mitochondrial membrane potential was determined by JC-1 staining. A nude mouse tumor assay was used to detect tumor formation. Hematoxylin and eosin (H&E) and immunohistochemical (IHC) staining were performed in tissue sections. Activation of Akt and Erk and expression of apoptosis-related proteins were determined by immunoblotting.<h4>Results</h4>ADM expression has been found up2022-01-01T00:00:00Z2022 Oct2025-04-19T11:32:37.824Z2025-04-19T11:32:37.824ZS-EPMC95269643618312310.1186/s12967-022-03647-6