{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kataki-Anastasakou A"],"funding":["National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["2124-2129"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9527085"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(11)"],"pubmed_abstract":["The widespread adoption of the bioorthogonal chemical reporter strategy revolutionized chemical biology. However, its translation to living mammals has been challenging, due to the size/stability properties of the chemical reporter group and/or the reaction kinetics of the labeling step. While developing new bioorthogonal reactions has been the traditional approach to optimizing the bioorthogonal chemical reporter strategy, here we present a different avenue, leveraging intermolecular interactions, to create bioorthogonal host-guest pairs. This approach, deemed \"bioorthogonal complexation, does not rely on activated functional groups or second-order rate constants. We utilize the cucurbit[7]uril (CB[7]) scaffold to showcase bioorthogonal complexation and determine that medium-affinity (<i>"],"journal":["ACS chemical biology"],"pubmed_title":["Cell-surface Labeling via Bioorthogonal Host-Guest Chemistry."],"pmcid":["PMC9527085"],"funding_grant_id":["1DP2GM13268","DP2 GM132680","R25 GM055052"],"pubmed_authors":["Sletten EM","Kataki-Anastasakou A","Hernandez S"],"additional_accession":[]},"is_claimable":false,"name":"Cell-surface Labeling via Bioorthogonal Host-Guest Chemistry.","description":"The widespread adoption of the bioorthogonal chemical reporter strategy revolutionized chemical biology. However, its translation to living mammals has been challenging, due to the size/stability properties of the chemical reporter group and/or the reaction kinetics of the labeling step. While developing new bioorthogonal reactions has been the traditional approach to optimizing the bioorthogonal chemical reporter strategy, here we present a different avenue, leveraging intermolecular interactions, to create bioorthogonal host-guest pairs. This approach, deemed \"bioorthogonal complexation, does not rely on activated functional groups or second-order rate constants. We utilize the cucurbit[7]uril (CB[7]) scaffold to showcase bioorthogonal complexation and determine that medium-affinity (<i>","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2025-04-22T19:06:09.649Z","creation":"2025-04-06T02:38:19.279Z"},"accession":"S-EPMC9527085","cross_references":{"pubmed":["34669367"],"doi":["10.1021/acschembio.1c00494"]}}