{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Johann DJ"],"funding":["The Kahlert Foundation","NCI NIH HHS","NIH Innovative Molecular Analysis Technologies"],"pagination":["643-658"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9527476"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["70(9)"],"pubmed_abstract":["Immunohistochemical (IHC) staining is an established technique for visualizing proteins in tissue sections for research studies and clinical applications. IHC is increasingly used as a targeting strategy for procurement of labeled cells via tissue microdissection, including immunodissection, computer-aided laser dissection (CALD), expression microdissection (xMD), and other techniques. The initial antigen retrieval (AR) process increases epitope availability and improves staining characteristics; however, the procedure can damage DNA. To better understand the effects of AR on DNA quality and quantity in immunodissected samples, both clinical specimens (<i>KRAS</i> gene mutation positive cases) and model system samples (lung cancer patient-derived xenograft tissue) were subjected to commonl"],"journal":["The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society"],"pubmed_title":["Effect of Antigen Retrieval on Genomic DNA From Immunodissected Samples."],"pmcid":["PMC9527476"],"funding_grant_id":["R33CA202811","R33 CA202811"],"pubmed_authors":["Shin IJ","Johann DJ","Roberge A","Tangrea MA","Liu M","Muesse J","Steliga MA","Peterson EA","Emmert-Buck MR","Laun S"],"additional_accession":[]},"is_claimable":false,"name":"Effect of Antigen Retrieval on Genomic DNA From Immunodissected Samples.","description":"Immunohistochemical (IHC) staining is an established technique for visualizing proteins in tissue sections for research studies and clinical applications. IHC is increasingly used as a targeting strategy for procurement of labeled cells via tissue microdissection, including immunodissection, computer-aided laser dissection (CALD), expression microdissection (xMD), and other techniques. The initial antigen retrieval (AR) process increases epitope availability and improves staining characteristics; however, the procedure can damage DNA. To better understand the effects of AR on DNA quality and quantity in immunodissected samples, both clinical specimens (<i>KRAS</i> gene mutation positive cases) and model system samples (lung cancer patient-derived xenograft tissue) were subjected to commonl","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-05-18T12:57:47.401Z","creation":"2025-05-18T12:57:47.401Z"},"accession":"S-EPMC9527476","cross_references":{"pubmed":["36129255"],"doi":["10.1369/00221554221124163"]}}