biostudies-literatureJohann DJThe Kahlert FoundationNCI NIH HHSNIH Innovative Molecular Analysis Technologies643-658https://www.ebi.ac.uk/biostudies/studies/S-EPMC9527476biostudies-literatureUnknown70(9)Immunohistochemical (IHC) staining is an established technique for visualizing proteins in tissue sections for research studies and clinical applications. IHC is increasingly used as a targeting strategy for procurement of labeled cells via tissue microdissection, including immunodissection, computer-aided laser dissection (CALD), expression microdissection (xMD), and other techniques. The initial antigen retrieval (AR) process increases epitope availability and improves staining characteristics; however, the procedure can damage DNA. To better understand the effects of AR on DNA quality and quantity in immunodissected samples, both clinical specimens (<i>KRAS</i> gene mutation positive cases) and model system samples (lung cancer patient-derived xenograft tissue) were subjected to commonlThe journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyEffect of Antigen Retrieval on Genomic DNA From Immunodissected Samples.PMC9527476R33CA202811R33 CA202811Shin IJJohann DJRoberge ATangrea MALiu MMuesse JSteliga MAPeterson EAEmmert-Buck MRLaun SfalseEffect of Antigen Retrieval on Genomic DNA From Immunodissected Samples.Immunohistochemical (IHC) staining is an established technique for visualizing proteins in tissue sections for research studies and clinical applications. IHC is increasingly used as a targeting strategy for procurement of labeled cells via tissue microdissection, including immunodissection, computer-aided laser dissection (CALD), expression microdissection (xMD), and other techniques. The initial antigen retrieval (AR) process increases epitope availability and improves staining characteristics; however, the procedure can damage DNA. To better understand the effects of AR on DNA quality and quantity in immunodissected samples, both clinical specimens (<i>KRAS</i> gene mutation positive cases) and model system samples (lung cancer patient-derived xenograft tissue) were subjected to commonl2022-01-01T00:00:00Z2022 Sep2025-05-18T12:57:47.401Z2025-05-18T12:57:47.401ZS-EPMC95274763612925510.1369/00221554221124163